Immunofluorescent analysis of FAST showing staining in the mitochondria of HeLa cells. HeLa cells were fixed in ice-cold MeOH for 5 min and stained using a FAST polyclonal antibody (Product # PA5-21449) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 346 and 549 of FAST (Uniprot ID#Q14296)|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA5-21449 targets FAST in ICC, IF, IHC (P), and WB applications and shows reactivity with Human, rat and mouse samples.
The PA5-21449 immunogen is a recombinant fragment corresponding to a region within amino acids 346 and 549 of FAST (Uniprot ID#Q14296).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Hepatitis B virus core protein inhibits Fas-mediated apoptosis of hepatoma cells via regulation of mFas/FasL and sFas expression.
PA5-21449 was used in western blot to describe the mechanisms by which hepatitis B virus core protein mediates resistance of hepatoma cells to apoptosis induced by anti-Fas antibody treatment
|Liu W,Lin YT,Yan XL,Ding YL,Wu YL,Chen WN,Lin X||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (29:1113)||2015|