Immunohistochemical analysis of FBL2 / FBXL2 in paraffin embedded Human Cerebral Cortex tissue. Steamed antigen retrieval was performed with citrate buffer pH6, followed by application of the FBL2 / FBXL2 antibody (Product # PA5-18181) (10 µg / ml) and AP-staining.
|Tested species reactivity||Human, Rat|
|Host / Isotype||Goat / IgG|
|Immunogen||Synthetic peptide sequence (VFSNNDEGLINKK) corresponding to the N-terminus amino acids of FBXL2|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||5-10 ug/ml|
|Western Blot (WB)||0.3-1.0 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with bovine, canine and rat based on sequence homology.
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs , which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains 12 tandem leucine-rich repeats. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
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