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| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:500 | - |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:50-1:200 | - |
Immunocytochemistry (ICC/IF) |
1:50-1:200 | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human |
Host/Isotype |
Rabbit / IgG |
Class |
Recombinant Monoclonal |
Type |
Antibody |
Clone |
JG35-83 |
Immunogen |
Recombinant protein within Human Emi1 aa 128-244 |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1 mg/mL |
Purification |
Protein A |
Storage buffer |
TBS, pH 7.4, with 40% glycerol, 0.05% BSA |
Contains |
0.05% sodium azide |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles, store in dark |
RRID |
AB_2848646 |
Positive Control: LOVO, Siha, Rat cerebellum tissue, human tonsil tissue, human colon cancer tissue, human kidney tissue, mouse cerebellum tissue.
F-box proteins, critical components of the conserved ubiquitin-protein ligase complex SCF (Skp1/Cdc53-Cullin1/F-box), recruit substrates for ubiquitination and consequent degradation through their specific protein-protein interaction domains. Novel human F-box proteins named FBG1, FBG2, FBG3, FBG4 and FBG5 constitute a subfamily of mammalian F-box proteins. FBG genes are expressed in a limited number of human tissues including kidney, liver, brain and muscle tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Early mitotic inhibitor 1; F-box only protein 5; F-box protein Fbx5
Gene Aliases: EMI1; FBX5; Fbxo31; FBXO5
UniProt ID: (Human) Q9UKT4
Entrez Gene ID: (Human) 26271
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