Invitrogen

FEN1 Monoclonal Antibody (A1B12)

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FEN1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of paraffin-embedded human breast carcinoma tissue using FEN1 Monoclonal Antibody (Product # MA5-42534). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the FEN1 antibody at a dilution of 1:400 for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

FEN1 Antibody in Western Blot (WB) — Western blot analysis of FEN1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. FEN1 Monoclonal Antibody (Product # MA5-42534) at 1:500 was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: MCF-7 cell lysate. Lane 2: K562 cell lysate. Lane 3: Daudi cell lysate.

FEN1 Antibody in Flow Cytometry (Flow) — Flow Cytometry analysis of FEN1 in K562 cells using FEN1 Monoclonal Antibody (Product # MA5-42534) at 1:50 (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1:1,000 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Product Details

MA5-42534

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:500	-
Flow Cytometry (Flow)	1:50-1:100	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	A1B12
Immunogen	Recombinant protein within human FEN1 aa 150-380 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	2 mg/mL
Purification	Protein G
Storage buffer	TBS, pH 7.4, with 50% glycerol
Contains	0.05% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Product Specific Information

Positive Control: MCF-7 cell lysate, K562 cell lysate, Daudi cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate, human tonsil tissue, human liver carcinoma tissue, human breast carcinoma tissue, K562.

Subcellular Location: Nucleolus, nucleoplasm. Mitochondrion.

Target Information

FEN-1 (Flap Endonuclease and Five prime Exonuclease) is a 42 a protein that functions as an endonuclease that cleaves branched DNA structures containing single-stranded 5' flaps or as an exonuclease on DNA nicks and gaps. FEN-1 endonuclease and exonuclease activities are stimulated by an association with proliferating cell nuclear antigen (PCNA) in the nucleus. FEN-1 is believed to have important roles in DNA replication and repair and is required in cell-free DNA replication systems.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DNase IV; FEN-1; FEN-1 {ECO:0000255|HAMAP-Rule:MF_03140}; FEN1; fen1 {ECO:0000255|HAMAP-Rule:MF_03140}; Flap endonuclease 1; flap endonuclease 1 {ECO:0000255|HAMAP-Rule:MF_03140}; Flap structure-specific endonuclease 1; flap structure-specific endonuclease 1 {ECO:0000255|HAMAP-Rule:MF_03140}; hFEN-1; Maturation factor 1; maturation factor-1; MF1

Gene Aliases: AW538437; FEN-1; FEN1; MF1; RAD2

UniProt ID: (Human) P39748, (Rat) Q5XIP6

Entrez Gene ID: (Human) 2237, (Mouse) 14156, (Rat) 84490

Molecular Function: exodeoxyribonuclease