Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:500-1:2,000 | - |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:100-1:500 | - |
Flow Cytometry (Flow) |
1:50-1:100 | - |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse, Rat |
Host/Isotype |
Mouse / IgG1 |
Class |
Monoclonal |
Type |
Antibody |
Clone |
A1B12 |
Immunogen |
Recombinant protein within human FEN1 aa 150-380 |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
2 mg/mL |
Purification |
Protein G |
Storage buffer |
TBS, pH 7.4, with 50% glycerol |
Contains |
0.05% sodium azide |
Storage conditions |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
Positive Control: MCF-7 cell lysate, K562 cell lysate, Daudi cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate, human tonsil tissue, human liver carcinoma tissue, human breast carcinoma tissue, K562.
Subcellular Location: Nucleolus, nucleoplasm. Mitochondrion.
FEN-1 (Flap Endonuclease and Five prime Exonuclease) is a 42 a protein that functions as an endonuclease that cleaves branched DNA structures containing single-stranded 5' flaps or as an exonuclease on DNA nicks and gaps. FEN-1 endonuclease and exonuclease activities are stimulated by an association with proliferating cell nuclear antigen (PCNA) in the nucleus. FEN-1 is believed to have important roles in DNA replication and repair and is required in cell-free DNA replication systems.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: DNase IV; FEN-1; FEN-1 {ECO:0000255|HAMAP-Rule:MF_03140}; FEN1; fen1 {ECO:0000255|HAMAP-Rule:MF_03140}; Flap endonuclease 1; flap endonuclease 1 {ECO:0000255|HAMAP-Rule:MF_03140}; Flap structure-specific endonuclease 1; flap structure-specific endonuclease 1 {ECO:0000255|HAMAP-Rule:MF_03140}; hFEN-1; Maturation factor 1; maturation factor-1; MF1
Gene Aliases: AW538437; FEN-1; FEN1; MF1; RAD2
UniProt ID: (Human) P39748, (Rat) Q5XIP6
Entrez Gene ID: (Human) 2237, (Mouse) 14156, (Rat) 84490
Molecular Function:
exodeoxyribonuclease
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn moreGet expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support