|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG|
|Immunogen||Purified recombinant fragment of FGF2 expressed in E. Coli.|
|Contains||0.03% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||1/200 - 1/1000|
|Western Blot (WB)||1/500 - 1/2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|ELISA (ELISA)||See 1 publications below|
MA5-15276 targets FGF2 in IHC and WB applications and shows reactivity with Human samples.
The MA5-15276 immunogen is purified recombinant fragment of FGF2 expressed in E. Coli.
The protein encoded by this gene is a member of the fibroblast growth factor family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
c-Myc-mediated repression of miR-15-16 in hypoxia is induced by increased HIF-2¿ and promotes tumor angiogenesis and metastasis by upregulating FGF2.
MA5-15276 was used in ELISA to study the role of elevated HIF-2alpha in the c-Myc-mediated repression of the miRNA-15-16 cluster that promotes tumor angiogenesis and metastasis
|Xue G,Yan HL,Zhang Y,Hao LQ,Zhu XT,Mei Q,Sun SH||Oncogene (34:1393)||2015|