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Western blot of FGF8/Fibroblast growth factor 8 in Jurkat lysate was performed using 35ug protein in RIPA buffer. Lysates were probed with a FGF8/Fibroblast growth factor 8 polyclonal antibody (Product# PA5-37860) at a dilution of 2ug/ml and incubated for 1 hour, followed by chemiluminescent detection.
|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Peptide with sequence C-HTTEQSLRFEFLNYP, from the internal region of FGF8|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with mouse, rat, dog and pig based on sequence homology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
AIGF; androgen-induced; androgen-induced growth factor; fibroblast growth factor 8; fibroblast growth factor 8 (androgen-induced); HBGF-8; heparin-binding growth factor 8; MGC149376
AIGF; FGF-8; FGF8; HBGF-8; HH6; KAL6