|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Non-human primate|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide, conjugated to KLH by a Glutaraldehyde linker, corresponding to residues K(809) L P Q Y P H I G S V K T(821) of the cytoplasmic region of Human FGFR2|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||15mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||1:2,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA1-24763 detects overexpressed FGFR2 from transfected human samples. This antibody shows no cross reactivity with FGFR1 or FGFR3. PA1-24763 is expected to cross react with mouse (92% conserved) and rat (92% conserved) due to sequence homology.
PA1-24763 has been successfully used in Immunohistochemistry, immunoprecipitation, and Western blot procedures.
The PA1-24763 immunogen is a synthetic peptide, conjugated to KLH by a Glutaraldehyde linker, corresponding to residues K(809) L P Q Y P H I G S V K T(821) of the cytoplasmic region of human FGFR2.
Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.
The protein encoded by this gene is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member is a high-affinity receptor for acidic, basic and/or keratinocyte growth factor, depending on the isoform. Mutations in this gene are associated with Crouzon syndrome, Pfeiffer syndrome, Craniosynostosis, Apert syndrome, Jackson-Weiss syndrome, Beare-Stevenson cutis gyrata syndrome, Saethre-Chotzen syndrome, and syndromic craniosynostosis. Multiple alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Non-human primate||Not Cited||
Therapeutic effect of nanogel-based delivery of soluble FGFR2 with S252W mutation on craniosynostosis.
PA1-24763 was used in immunoprecipitation and western blot to study the mechanisms of craniosynostosis and the effects of purified soluble FGFR2 harboring the S252W mutation on the calvarial sutures in Apert syndrome mice
|Yokota M,Kobayashi Y,Morita J,Suzuki H,Hashimoto Y,Sasaki Y,Akiyoshi K,Moriyama K||PloS one (9:null)||2014|