|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide, conjugated to KLH by a Glutaraldehyde linker, corresponding to residues A(362) P G R E K E I T A S P D K(374) of human FGFR2.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||15mM sodium azide|
|Storage Conditions||-20°C or -80°C if preferred|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:1000|
|Western Blot (WB)||1:2,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-24764 detects overexpressed FGFR2 from transfected human samples. This antibody shows no cross reactivity with FGFR1 or FGFR3. PA1-24764 is expected to cross react with mouse (92% conserved) and rat (92% conserved) due to sequence homology.
PA1-24764 has been successfully used in immunoprecipitation, immunohistochemistry (paraffin), and Western blot procedures. The molecular weight is 110 kDa.
The PA1-24764 immunogen is a synthetic peptide, conjugated to KLH by a Glutaraldehyde linker, corresponding to residues A(362) P G R E K E I T A S P D K(374) of human FGFR2.
Avoid freeze/thaw cycles
The protein encoded by this gene is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member is a high-affinity receptor for acidic, basic and/or keratinocyte growth factor, depending on the isoform. Mutations in this gene are associated with Crouzon syndrome, Pfeiffer syndrome, Craniosynostosis, Apert syndrome, Jackson-Weiss syndrome, Beare-Stevenson cutis gyrata syndrome, Saethre-Chotzen syndrome, and syndromic craniosynostosis. Multiple alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.