Immunofluorescent analysis using a FGFR2 polyclonal antibody (Product # PA5-14651) on FGFR2/isolectinB4 (C) and FGFR1/isolectinB4 (D) staining of apparent mesenchymal cells and the subpopulation of endothelial cells. Virtually all other dispersed apparent mesenchymal cells express FGFR1 and FGFR2 (merged image in E). F: FGFR2 (F) and FGFR1 (G) staining in clustered cells of epithelial origin (inferred by morphology here) demonstrating that epithelial cells express both FGFR1 and FGFR2 (merged image with DAPI staining in H).
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLH conjugated synthetic peptide between 22-51 amino acids from the N-terminal region of human FGFR2|
|Purification||Protein A, Affinity chromatography|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:10-1:50|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
FGFR2 is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member is a high-affinity receptor for acidic, basic and/or keratinocyte growth factor, depending on the isoform. Mutations in the gene are associated with many craniosynostotic syndromes and bone malformations. The genomic organization of the gene encompasses 20 exons. Alternative splicing in multiple exons, including those encoding the Ig-like domains, the transmembrane region and the carboxyl terminus, results in varied isoforms which differ in structure and specificity. Isoform 1 has equal affinity for aFGF and bFGF but does not bind KGF.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.