OriGene

FGFR3 Monoclonal Antibody (OTI1B10), TrueMAB™

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated. View Details

View all (25) FGFR3 antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

Product Image Gallery

FGFR3 Antibody in Immunofluorescence (IF) — Figure from citation: Immunohistochemical and Immunof uorscense staining with anti-BGN and anti-FGFR3 of serial sections of adult human testis tissue with full spermatogenesis and fetal bone sample (gestational week 10). Scale bar is 50 um for all images. Dilution: 1:200 View Citation

FGFR3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-FGFR3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801078)

FGFR3 Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FGFR3 (RC215533, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-FGFR3. Positive lysates LY424902 (100 µg) and LC424902 (20 µg) can be purchased separately from OriGene.

FGFR3 Antibody in Knockout — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Loss of signal was observed for target protein in FGFR3 knockout (KO) 293T cell line using Anti-FGFR3 monoclonal antibody (Product # CF801078). {KO}

Product Details

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:150

Western Blot (WB)

1:2000

Product Specifications

Species Reactivity

Human

Host / Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI1B10

Immunogen

Human recombinant protein fragment corresponding to amino acids 23-375 of human FGFR3 produced in SF9 cell.

Conjugate

Unconjugated

Form

lyophilized

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Reconstitute with PBS (pH 7.3) and recommend to perform another round of desalting process using Product # 7KMWCO

Target Information

This gene encodes a member of the fibroblast growth factor receptor (FGFR) family, with its amino acid sequence being highly conserved between members and among divergent species. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds acidic and basic fibroblast growth hormone and plays a role in bone development and maintenance. Mutations in this gene lead to craniosynostosis and multiple types of skeletal dysplasia. Three alternatively spliced transcript variants that encode different protein isoforms have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD333; FGFR-3; FGFR3deltaTM; Fibroblast growth factor receptor 3; fibroblast growth factor receptor 3 variant 4; hydroxyaryl-protein kinase; tyrosine kinase JTK4

Gene Aliases: ACH; CD333; CEK2; FGFR3; HSFGFR3EX; JTK4

UniProt ID: (Human) P22607

Entrez Gene ID: (Human) 2261

Function(s)