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FHL2 Antibody (MA5-38085) in WB
Western blot analysis of FHL2 in extracts of various cell lines. Samples were incubated with FHL2 Monoclonal antibody (Product # MA5-38085) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3 min.
Product Details
MA5-38085
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
ARC2068
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 50-260 of human FHL2 (Q14192)
Conjugate
Unconjugated
Form
Liquid
Concentration
0.8 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2898003
Product Specific Information
Positive Samples: Mouse heart, Rat heart, SKOV3, RD
Immunogen sequence: DCKDLSYKDR HWHEACFHCS QCRNSLVDKP FAAKEDQLLC TDCYSNEYSS KCQECKKTIM PGTRKMEYKG SSWHETCFIC HRCQQPIGTK SFIPKDNQNF CVPCYEKQHA MQCVQCKKPI TTGGVTYREQ PWHKECFVCT ACRKQLSGQR FTARDDFAYC LNCFCDLYAK KCAGCTNPIS GLGGTKYISF EERQWHNDCF NCKKCSLSLV G
Target Information
This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. This protein is thought to have a role in the assembly of extracellular membranes. Also, this gene is down-regulated during transformation of normal myoblasts to rhabdomyosarcoma cells and the encoded protein may function as a link between presenilin-2 and an intracellular signaling pathway. Multiple alternatively spliced variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: aging-associated gene 11; down-regulated in rhabdomyosarcoma LIM protein; FHL-2; Four and a half LIM domains protein 2; LIM domain protein DRAL; OTTHUMP00000203164; OTTHUMP00000203168; OTTHUMP00000203169; Skeletal muscle LIM-protein 3; SLIM 3; SLIM-3
Gene Aliases: AAG11; DRAL; FHL-2; FHL2; SLIM-3; SLIM3
UniProt ID: (Human) Q14192, (Mouse) O70433, (Rat) O35115
Entrez Gene ID: (Human) 2274, (Mouse) 14200, (Rat) 63839
negative regulation of transcription from RNA polymerase II promoter
osteoblast differentiation
transcription, DNA-templated
response to hormone
androgen receptor signaling pathway
negative regulation of apoptotic process
positive regulation of transcription, DNA-templated
atrial cardiac muscle cell development
ventricular cardiac muscle cell development
heart trabecula formation
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
negative regulation of transcription, DNA-templated
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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