MA1-40200 detects FHL2 from human, mouse, and rat samples.
MA1-40200 has been successfully used in immunohistochemistry (frozen), immunohistochemistry (paraffin), Western blot, and immunofluorescence applications.
The MA1-40200 immunogen is human FHL2.
MA1-40200 recognizes the N-terminal Zn-Finger motif, it does not crossreact in Western Blotting with the FHL1 and FHL3 proteins. MA1-40200 antibody is cross reactive with mouse and rat FHL2.
LIM proteins contain a highly conserved double zinc finger motif called the LIM domain. Five proteins share the same structural organization and a high degree of sequence homology in this group: four and a half LIM domain protein (FHL) 1, FHL2, FHL3, FHL4, and activator of cAMP-responsive element (CRE) modulator (CREM) in testis (ACT). LIM domains are constituted by a conserved cysteine- and histidine-rich structure shaped in two repeated zinc fingers first identified in the proteins encoded by the Lin-11, Isl-1, and Mec-3 genes. The LIM domain has been shown to function as a protein–protein interaction domain, and has often been described in association with other functional protein motifs, such as homeobox and kinase domains. FHL2 seems to be a promiscuous coactivator because it modulates the activity of the androgen receptor, CRE-binding protein (CREB), and WT1, although some degree of specificity is present because it is unable to stimulate CREM- and Sp1-dependent transcription. FHL2 expression was originally described to be restricted to the heart but it is inducible in other cell types. FHL2 shows specific interaction with betacatenin, which requires the intact structure of all four LIM domains of FHL2 and the N-terminus plus the first armadillo repeat region of beta-catenin. FHL2 is a muscle-specific repressor of LEF/TCF target genes and promotes myogenic differentiation by interacting with beta-catenin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: aging-associated gene 11; down-regulated in rhabdomyosarcoma LIM protein; FHL-2; Four and a half LIM domains protein 2; Four-and-a-half LIM domains 2; LIM domain protein DRAL; Skeletal muscle LIM-protein 3; SLIM 3; SLIM-3
Gene Aliases: AAG11; DRAL; FHL-2; FHL2; SLIM-3; SLIM3