|Tested species reactivity||Mouse, Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues C(80) Q G P K G K D A P F K D S E(94) of mouse FIAF.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA1-1053 detects fasting-induced adipose factor (FIAF) from rat and mouse adipose tissue.
PA1-1053 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~50 kDa protein representing FIAF from rat adipose tissue extracts.
The PA1-1053 immunogen is a synthetic peptide corresponding to residues C(80) Q G P K G K D A P F K D S E(94) of mouse FIAF.
Adipose tissue is an energy reserve in animals and, in nondomestic species, is strictly regulated. Adipose cells produce and secrete numerous physiologically important proteins that help regulate whole body metabolism, such as leptin and Acrp30. Fasting causes significant changes in nutrient metabolism and many of these changes are controlled by transcription factors that regulate rate-limiting enzymes. An important transcription factor that mediates metabolic processes induced by fasting is peroxisome proliferator-activated receptor alpha (PPAR alpha). PPAR alpha has recently been reported to target a novel gene encoding the secreted protein FIAF (fasting-induced adipose factor). FIAF is expressed predominantly in adipose tissue and is strongly upregulated in white adipose tissue and the liver under fasting conditions. Initial studies suggest that FIAF represents a novel endocrine signal helping to regulate metabolism, especially under fasting conditions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Characterization of the fasting-induced adipose factor FIAF, a novel peroxisome proliferator-activated receptor target gene.
PA1-1053 was used in western blot to investigate the function of PPAR alpha and characterize FIAF during fasting
|Kersten S,Mandard S,Tan NS,Escher P,Metzger D,Chambon P,Gonzalez FJ,Desvergne B,Wahli W||The Journal of biological chemistry (275:28488)||2000|