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Product Details
MA5-35336
| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:500-1:2,000 | - |
Immunohistochemistry (IHC) |
1:50-1:200 | - |
Immunocytochemistry (ICC/IF) |
1:50-1:200 | - |
Immunoprecipitation (IP) |
1:500-1:1000 | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human, Mouse, Rat |
Host/Isotype |
Rabbit / IgG |
Class |
Recombinant Monoclonal |
Type |
Antibody |
Clone |
3S3J1 |
Immunogen |
A recombinant fusion protein containing a sequence corresponding to amino acids 1-122 of human FIS1. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1 mg/mL |
Purification |
Affinity Chromatography |
Storage buffer |
PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
Contains |
0.01% thimerosal |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles |
RRID |
AB_2849238 |
Target Information
Fis1, a 17KDa integral membrane protein, is a novel member of mitochondrial fission machinery. It consists of a central leucine-zipper domain, a coiled-coil region, a tetratricopeptide repeat domain and a C-terminal transmembrane domain. It is a human orthologue of Fis1p (S.cerevisiae) and is localized in the outer mitochondrial membrane. Fis1 acts as a pro-apoptotic factor and plays a vital role in the regulation of mitochondrial fission. It interacts with DLP1 and Mdv1p and facilitates Dnm1p recruitment to the functional fission complex, thereby inducing apoptosis. Role of Fis1 has also been implicated in growth and division of peroxisomes. Loss of Fis1 inhibits apoptosis and results in elongation of mammalian mitochondria.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FIS1 homolog; fis1 {ECO:0000250|UniProtKB:Q9Y3D6}; fission 1 (mitochondrial outer membrane) homolog; H_NH0132A01.6; hFis1; Mitochondrial fission 1 protein; mitochondrial fission molecule; rFis1; tetratricopeptide repeat domain 11; Tetratricopeptide repeat protein 11; TPR repeat protein 11
Gene Aliases: 2010003O14Rik; CGI-135; FIS1; TTC11
UniProt ID: (Human) Q9Y3D6, (Rat) P84817, (Mouse) Q9CQ92
Entrez Gene ID: (Human) 51024, (Rat) 288584, (Mouse) 66437
mitochondrial fission
mitophagy
release of cytochrome c from mitochondria
protein targeting to mitochondrion
positive regulation of cytosolic calcium ion concentration
mitochondrial fusion
regulation of mitochondrion organization
peroxisome fission
negative regulation of endoplasmic reticulum calcium ion concentration
calcium-mediated signaling using intracellular calcium source
positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
mitochondrial fragmentation involved in apoptotic process
protein homooligomerization
positive regulation of mitochondrial calcium ion concentration
mitochondrion morphogenesis
positive regulation of mitochondrial fission
positive regulation of protein targeting to membrane
positive regulation of intrinsic apoptotic signaling pathway
apoptotic process
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