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|Tested species reactivity||Chem, Tag|
|Host / Isotype||Mouse / IgG1, kappa|
|Storage buffer||PBS, pH 7.6, with 15mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||1:50 - 1:200|
|Immunocytochemistry (ICC)||1:50 - 1:200|
|Immunofluorescence (IF)||1:50 - 1:200|
|Immunohistochemistry (IHC)||1:50 - 1:200|
|Immunoprecipitation (IP)||1:50 - 1:200|
|Western Blot (WB)||1:50 - 1:200|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.
Fluorophore: Tetramethyl rodamine 5 (and 6)-isothiocyanate (TRITC)
Amax = 550nm; Emax = 570 nm
Fluorophore/Protein Absorbance Ratio: A550/A280 = 0.44
Product #31687 is recommended for use in ELISA, immunocytochemical, immunofluorescent, immunohistochemical, immunoprecipitation and Western blot applications.
Restoration and Storage: Store product at 4°C until opened. Restore with 0.35 ml distilled water. Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature. Product may be stored for up to 6 weeks at 4°C as an undiluted liquid. After dilution, do not use for more than one day. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of 50% glycerol and store at -20°C; or as an alternative, aliquot without glycerol and store at -80° or below, avoiding repeated freezing and thawing.
Country of Origin: USA
FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.