Fixed and permeabilized A549 cells were labeled with Mouse Tubulin Antibody (1 µg/ml), followed by FITC Goat Anti-Mouse IgG (right peak, green). To confirm specificity, the cells were labeled with a Mouse Isotype control and stained using FITC Goat Anti-Mouse IgG (middle peak, pink). Quenching of fluorescence upon preincubation of 1:100 of ABfinity™ Recombinant FITC Rabbit Monoclonal Antibody (701078) is shown as middle peak, red. Unstained control cells are as shown (left peak, purple). The representative 10,000 cells were obtained and analyzed for each sample using Attune® Acoustic Focusing Cytometer.
|Tested species reactivity||Chemical, Tag|
|Host / Isotype||Rabbit / IgG|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1µg/ml|
|Fluorescent Quenching (FQ)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Fluorescein isothiocyanate (FITC) is a fluorescein derived reagent having an isothiocyanate functional group attached to the core ring structure. FITC is one of the most popular reagents for fluorescent labeling of biomolecules and is used mainly in immunoflouresence studies. Anti-FITC antibodies can be used for isolating FITC labeled cells by magnetic microbeads based sorting, as well as removal of background signal.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.