|Tested species reactivity||Virus|
|Host / Isotype||Mouse / IgG1|
|Storage buffer||PBS, pH 7.2|
|Contains||0.05% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The specific epitope recognized by clone PAK3-2C1 appears to be enclosed in a tight peptide coil during gag production and maturation. The addition of 0.3% Triton-X detergent is recommended to reveal this epitope for antibody binding.
Membrane permeabilization is required for flow cytometry applications. For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
The lentivirus FIV is responsible for a progressive and debilitating immunodeficiency syndrome in domestic cats, similar to that caused by HIV. It is a complex retrovirus with a tightly-packed genome containing the structrual genes gag, env and pro-pol, as well as accessory genes vif, rev and ORF-A/2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.