Immunofluorescent analysis of Heat Shock protein 56 (HSP56, green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed without (left panel) or with (right panel) a HSP56 polyclonal antibody (Product # PA3-020) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on Thermo Scientific ArrayScan or ToxInsight Instrument at 20X magnification.
|Tested species reactivity||Human, Mouse, Non-human primate, Rabbit|
|Published species reactivity||Rat, Non-human primate, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues C(441) K D E R N D V A G S Q S Q V E T E A(458) of rabbit Heat Shock Protein 56.|
|Purification||Antigen affinity chromatography|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA3-020 detects heat shock protein 56 (HSP56), also known as FKBP4/FKBP52, from human, monkey, mouse and rabbit tissues.
PA3-020 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects a prominent ~52 kDa band representing HSP56 HeLa cell lysate.
The PA3-020 immunogen is a synthetic peptide corresponding to residues C(441) K D E R N D V A G S Q S Q V E T E A(458) of rabbit Heat Shock Protein 56.
Reconstitute with PBS.
Heat shock proteins (HSP) are expressed in response to various biological stresses, including high temperatures. There are several major families of HSPs including HSP70, HSP90 and HSP100.
Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation, providing a possible mechanism of immunosuppression by CsA and FK506. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase's, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
FK506-binding protein 52 is essential to uterine reproductive physiology controlled by the progesterone receptor A isoform.
PA3-020 was used in western blot to study the role of FKBP52 in progesterone receptor action.
|Yang Z,Wolf IM,Chen H,Periyasamy S,Chen Z,Yong W,Shi S,Zhao W,Xu J,Srivastava A,Sánchez ER,Shou W||Molecular endocrinology (Baltimore, Md.) (20:2682)||2006|
|Non-human primate||Not Cited||
Progesterone receptor structure and function altered by geldanamycin, an hsp90-binding agent.
PA3-020 was used in western blot to investigate the assembly pathway of progesterone receptor complexes
|Smith DF,Whitesell L,Nair SC,Chen S,Prapapanich V,Rimerman RA||Molecular and cellular biology (15:6804)||1995|
Protection against ischemic brain damage in rats by immunophilin ligand GPI-1046.
PA3-020 was used in immunohistochemistry to evaluate the protective effect of immunophilin ligand GPI-1046 against ischemic brain damage in rats
|Li F,Omori N,Hayashi T,Jin G,Sato K,Nagano I,Shoji M,Abe K||Journal of neuroscience research (76:383)||2004|
Early decrease of the immunophilin FKBP 52 in the spinal cord of a transgenic model for amyotrophic lateral sclerosis.
PA3-020 was used in immunohistochemistry to investigate the effect of the downregulation of FKBP-52 on the pathogenesis of amyotrophic lateral sclerosis
|Manabe Y,Warita H,Murakami T,Shiote M,Hayashi T,Omori N,Nagano I,Shoji M,Abe K||Brain research (935:124)||2002|