|Tested species reactivity||Tag|
|Published species reactivity||Rat, Non-human primate, Human, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide: D Y K D D D D K C|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||1 ug/ml|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|ChIP assay (ChIP)||See 1 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Immunohistochemistry (IHC)||See 1 publications below|
|Immunocytochemistry (ICC)||See 4 publications below|
|Western Blot (WB)||See 4 publications below|
|Gel Shift (GS)||See 1 publications below|
PA1-984B detects the DYKDDDDK epitope tag sequence, similar to the FLAG tag from Sigma.
PA1-984B has been successfully used in Western blot and immunofluorescence procedures to detect the presence of fusion proteins containing the epitope tag sequence DYKDDDDK.
PA1-984B immunizing peptide corresponds to the epitope tag sequence DYKDDDDKC. This peptide (Cat. # PEP-087) is available for use in neutralization and control experiments.
Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. To utilize this feature, protein expression vectors are typically engineered with a nucleotide sequence that encodes the peptide epitope tag. The gene of interest is cloned in-frame relative to the tag and, upon expression, the protein of interest is synthesized as a fusion protein with the peptide tag. Fusion protein detection and/or purification is mediated by highly specific antibodies to the engineered peptide, thus eliminating the need for antibodies to proteins from each newly cloned gene. Commonly used epitope tags include glutathione-S-transferase (GST), c-myc, 6-histidine (6X-His), FLAG®, green fluorescent protein (GFP), maltose binding protein (MBP), influenza A virus haemagglutinin (HA), b-galactosidase, and GAL4.
FLAG® and anti-FLAG® are registered trademarks of Sigma-Aldrich Biotechnology Co.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Selective regulation of Mmp13 by 1,25(OH)2D3, PTH, and Osterix through distal enhancers.
PA1-984B was used in ChIP assay to define the specific roles of distal enhancers in the regulation of Mmp13 gene expression
|Meyer MB,Benkusky NA,Onal M,Pike JW||The Journal of steroid biochemistry and molecular biology (164:258)||2016|
Stability of HIB-Cul3 E3 ligase adaptor HIB Is Regulated by Self-degradation and Availability of Its Substrates.
PA1-984B was used in immunohistochemistry to investigate the regulation of HIB
|Zhou Z,Xu C,Chen P,Liu C,Pang S,Yao X,Zhang Q||Scientific reports (5:null)||2015|
The production of viral vectors designed to express large and difficult to express transgenes within neurons.
PA1-984B was used in immunohistochemistry to describe the generation of adeno-associated viruses and lentiviruses designed to express GluN2A or GluN2B subunits of the N-methyl-D-aspartate receptor in neurons
|Holehonnur R,Lella SK,Ho A,Luong JA,Ploski JE||Molecular brain (8:null)||2015|
Palmitoylation of TNF alpha is involved in the regulation of TNF receptor 1 signalling.
PA1-984B was used in immunocytochemistry to study the regulation of TNFR1 signaling by palmitoylation of TNFalpha
|Poggi M,Kara I,Brunel JM,Landrier JF,Govers R,Bonardo B,Fluhrer R,Haass C,Alessi MC,Peiretti F||Biochimica et biophysica acta (1833:602)||2013|
Cysteine-scanning mutagenesis of serotonin transporter intracellular loop 2 suggests an alpha-helical conformation.
PA1-984B was used in immunocytochemistry to demonstrate a possible mechanism for the regulation of SERT activity.
|Zhang YW,Rudnick G||The Journal of biological chemistry (280:30807)||2005|
Pharmacological rescue of trafficking defective HERG channels formed by coassembly of wild-type and long QT mutant N470D subunits.
PA1-984B was used in immunocytochemistry to investigate defective HERG channels formed by N470D mutant and WT subunits and the rescue by the blocking drug E-4031
|Gong Q,Anderson CL,January CT,Zhou Z||American journal of physiology. Heart and circulatory physiology (287:H652)||2004|
Differences in endosomal targeting of human (beta)1- and (beta)2-adrenergic receptors following clathrin-mediated endocytosis.
PA1-984B was used in immunocytochemistry to study the internalization of adrenergic receptors.
|Liang W,Curran PK,Hoang Q,Moreland RT,Fishman PH||Journal of cell science (117:723)||2004|
|Not Applicable||Not Cited||
c-Abl tyrosine kinase regulates serum-induced nuclear export of diacylglycerol kinase ¿ by phosphorylation at Tyr-218.
PA1-984B was used in western blot to investigate the regulation of nuclear export of diacylglycerol kinase alpha by c-Abl tyrosine kinase
|Matsubara T,Ikeda M,Kiso Y,Sakuma M,Yoshino K,Sakane F,Merida I,Saito N,Shirai Y||The Journal of biological chemistry (287:5507)||2012|
Transcriptional control of BubR1 by p53 and suppression of centrosome amplification by BubR1.
PA1-984B was used in western blot to study the regulatory control of BubR1 and the effect of BubR1 on centrosome amplification by BubR1.
|Oikawa T,Okuda M,Ma Z,Goorha R,Tsujimoto H,Inokuma H,Fukasawa K||Molecular and cellular biology (25:4046)||2005|
|Non-human primate||Not Cited||
Critical role for protein phosphatase 2A heterotrimers in mammalian cell survival.
PA1-984B was used in western blot to investigate the effect of protein phosphatase 2A on mammalian cell survial.
|Strack S,Cribbs JT,Gomez L||The Journal of biological chemistry (279:47732)||2004|
Multiple type A/B heterogeneous nuclear ribonucleoproteins (hnRNPs) can replace hnRNP A1 in mouse hepatitis virus RNA synthesis.
PA1-984B was used in western blot to investigate the interaction of CB3 intracellular proteins with mouse hepatitis virus RNA
|Shi ST,Yu GY,Lai MM||Journal of virology (77:10584)||2003|
Unregulated smooth-muscle myosin in human intestinal neoplasia.
PA1-984B was used in EMSA assay to investigate the changes of myh11 in human intestinal tumor
|Alhopuro P,Phichith D,Tuupanen S,Sammalkorpi H,Nybondas M,Saharinen J,Robinson JP,Yang Z,Chen LQ,Orntoft T,Mecklin JP,Järvinen H,Eng C,Moeslein G,Shibata D,Houlston RS,Lucassen A,Tomlinson IP,Launonen V,Ristimäki A,Arango D,Karhu A,Sweeney HL,Aaltonen LA||Proceedings of the National Academy of Sciences of the United States of America (105:5513)||2008|