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|Tested species reactivity||Tag|
|Published species reactivity||Rat, Primate, Human, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide: D Y K D D D D K C|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||1 ug/ml|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-984B detects the DYKDDDDK epitope tag sequence, similar to the FLAG tag from Sigma.
PA1-984B has been successfully used in Western blot and immunofluorescence procedures to detect the presence of fusion proteins containing the epitope tag sequence DYKDDDDK.
PA1-984B immunizing peptide corresponds to the epitope tag sequence DYKDDDDKC. This peptide (Cat. # PEP-087) is available for use in neutralization and control experiments.
Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. To utilize this feature, protein expression vectors are typically engineered with a nucleotide sequence that encodes the peptide epitope tag. The gene of interest is cloned in-frame relative to the tag and, upon expression, the protein of interest is synthesized as a fusion protein with the peptide tag. Fusion protein detection and/or purification is mediated by highly specific antibodies to the engineered peptide, thus eliminating the need for antibodies to proteins from each newly cloned gene. Commonly used epitope tags include glutathione-S-transferase (GST), c-myc, 6-histidine (6X-His), FLAG®, green fluorescent protein (GFP), maltose binding protein (MBP), influenza A virus haemagglutinin (HA), b-galactosidase, and GAL4.
FLAG® and anti-FLAG® are registered trademarks of Sigma-Aldrich Biotechnology Co.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The production of viral vectors designed to express large and difficult to express transgenes within neurons.
PA1-984B was used in immunohistochemistry to describe the generation of adeno-associated viruses and lentiviruses designed to express GluN2A or GluN2B subunits of the N-methyl-D-aspartate receptor in neurons
|Holehonnur R,Lella SK,Ho A,Luong JA,Ploski JE||Molecular brain (8:null)||2015|
Palmitoylation of TNF alpha is involved in the regulation of TNF receptor 1 signalling.
PA1-984B was used in immunocytochemistry to study the regulation of TNFR1 signaling by palmitoylation of TNFalpha
|Poggi M,Kara I,Brunel JM,Landrier JF,Govers R,Bonardo B,Fluhrer R,Haass C,Alessi MC,Peiretti F||Biochimica et biophysica acta (1833:602)||2013|
Cysteine-scanning mutagenesis of serotonin transporter intracellular loop 2 suggests an alpha-helical conformation.
PA1-984B was used in immunocytochemistry to demonstrate a possible mechanism for the regulation of SERT activity.
|Zhang YW,Rudnick G||The Journal of biological chemistry (280:30807)||2005|
Pharmacological rescue of trafficking defective HERG channels formed by coassembly of wild-type and long QT mutant N470D subunits.
PA1-984B was used in immunocytochemistry to investigate defective HERG channels formed by N470D mutant and WT subunits and the rescue by the blocking drug E-4031
|Gong Q,Anderson CL,January CT,Zhou Z||American journal of physiology. Heart and circulatory physiology (287:H652)||2004|
Differences in endosomal targeting of human (beta)1- and (beta)2-adrenergic receptors following clathrin-mediated endocytosis.
PA1-984B was used in immunocytochemistry to study the internalization of adrenergic receptors.
|Liang W,Curran PK,Hoang Q,Moreland RT,Fishman PH||Journal of cell science (117:723)||2004|
|Not Applicable||Not Cited||
c-Abl tyrosine kinase regulates serum-induced nuclear export of diacylglycerol kinase α by phosphorylation at Tyr-218.
PA1-984B was used in western blot to investigate the regulation of nuclear export of diacylglycerol kinase alpha by c-Abl tyrosine kinase
|Matsubara T,Ikeda M,Kiso Y,Sakuma M,Yoshino K,Sakane F,Merida I,Saito N,Shirai Y||The Journal of biological chemistry (287:5507)||2012|
Transcriptional control of BubR1 by p53 and suppression of centrosome amplification by BubR1.
PA1-984B was used in western blot to study the regulatory control of BubR1 and the effect of BubR1 on centrosome amplification by BubR1.
|Oikawa T,Okuda M,Ma Z,Goorha R,Tsujimoto H,Inokuma H,Fukasawa K||Molecular and cellular biology (25:4046)||2005|
Critical role for protein phosphatase 2A heterotrimers in mammalian cell survival.
PA1-984B was used in western blot to investigate the effect of protein phosphatase 2A on mammalian cell survial.
|Strack S,Cribbs JT,Gomez L||The Journal of biological chemistry (279:47732)||2004|
Multiple type A/B heterogeneous nuclear ribonucleoproteins (hnRNPs) can replace hnRNP A1 in mouse hepatitis virus RNA synthesis.
PA1-984B was used in western blot to investigate the interaction of CB3 intracellular proteins with mouse hepatitis virus RNA
|Shi ST,Yu GY,Lai MM||Journal of virology (77:10584)||2003|
Unregulated smooth-muscle myosin in human intestinal neoplasia.
PA1-984B was used in EMSA assay to investigate the changes of myh11 in human intestinal tumor
|Alhopuro P,Phichith D,Tuupanen S,Sammalkorpi H,Nybondas M,Saharinen J,Robinson JP,Yang Z,Chen LQ,Orntoft T,Mecklin JP,Järvinen H,Eng C,Moeslein G,Shibata D,Houlston RS,Lucassen A,Tomlinson IP,Launonen V,Ristimäki A,Arango D,Karhu A,Sweeney HL,Aaltonen LA||Proceedings of the National Academy of Sciences of the United States of America (105:5513)||2008|
DDDDK; DDDDK epitope tag; DYKDDDDK epitope tag; DYKDDDDK tag; ECS epitope tag; ECS tag; Enterokinase Cleavage Site epitope tag; Enterokinase Cleavage Site tag; FLAG antibody; FLAG Tag