Invitrogen

FOXA1 Recombinant Rabbit Monoclonal Antibody (JF10-02)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

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Datasheet

Protocols

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Antibody Testing Data (9)
Advanced Verification (2)

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FOXA1 Antibody (MA5-32556) in WB

Western blot was performed using Anti-FOXA1 Recombinant Rabbit Monoclonal Antibody (JF10-02) (Product # MA5-32556) and a 49 kDa band corresponding to FOXA1 was observed in MCF7, T-47D, LNCaP and SW480 which are reported to be positive cell lines but not in other cell lines HCT 116 and SH-SY5Y. Nuclear enriched extracts (30 µg lysate) of MCF7 (Lane 1), T-47D (Lane 2), LNCaP (Lane 3), SW480 (Lane 4), HCT 116 (Lane 5) and SH-SY5Y (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins we... View More

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FOXA1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of FOXA1 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with FOXA1 Recombinant Rabbit Monoclonal Antibody (JF10-02) (Product # MA5-32556) at 1:50-1:200 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained withRhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents negative cell line HCT 116 having no signal . Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

FOXA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of FOXA1 of paraffin-embedded Human breast carcinoma tissue using a FOXA1 Monoclonal antibody (Product #MA5-32556). Counter stained with hematoxylin.

FOXA1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across MCF7, T-47D, LNCaP, SW480, HCT-116 and SHSY-5Y owing to their inherent genetic constitution. Relative expression of Ci-fkh; fkd7; fkh; fork head domain; forkhead box A1; forkhead box protein A1; forkhead-7; Foxa1; FoxA4a; hepatocyte nuclear factor 3 alpha (winged helix transcription factor); hepatocyte nuclear factor 3, alpha; hepatocyte nuclear factor 3-alpha; HNF3A; HNF-3A; HNF3alpha; HNF-3-alpha; HNF3-alpha; MGC33105; MGC52590; MGC75967; pintallavis; TCF3A; Tcf-3a; tFoxA1; Transcription factor 3A; xfkh1; xfkh2 was observed in MCF7, T-47D, LNCaP and SW480 using Anti-FOXA1 Recombinant Rabbit Monoclonal Antibody (JF10-02) (Product # MA5-32556) in Western Blot. {RE}

Product Details

MA5-32556

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:2,000

Immunohistochemistry (IHC)

1:50-1:200

Immunocytochemistry (ICC/IF)

1:50-1:200

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JF10-02

Immunogen

Recombinant protein within Human FOXA1 aa 320-472

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2809833

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Ci-fkh; fkd7; fkh; fork head domain; Forkhead box protein A1; forkhead-7; FoxA4a; hepatocyte nuclear factor 3 alpha (winged helix transcription factor); hepatocyte nuclear factor 3, alpha; Hepatocyte nuclear factor 3-alpha; HNF-3-alpha; HNF-3A; HNF3-alpha; HNF3alpha; MGC33105; MGC52590; MGC75967; pintallavis; TCF-3A; Transcription factor 3A; xfkh1; xfkh2

Gene Aliases: FOXA1; Hnf-3a; HNF3A; Tcf-3a; TCF3A

UniProt ID: (Human) P55317, (Mouse) P35582, (Rat) P23512

Entrez Gene ID: (Human) 3169, (Mouse) 15375, (Rat) 25098

Molecular Function: winged helix/forkhead transcription factor