OriGene

FOXA1 Monoclonal Antibody (OTI3A8), TrueMAB™

View all (21) FOXA1 antibodies

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Questions & Answers

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FOXA1 Antibody in Immunofluorescence (IF) — Anti-FOXA1 mouse monoclonal antibody (TA500245) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FOXA1(RC206045).

FOXA1 Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOXA1 (RC206045, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-FOXA1. Positive lysates LY401427 (100 µg) and LC401427 (20 µg) can be purchased separately from OriGene.

FOXA1 Antibody in Western Blot (WB) — Western blot analysis of extracts (35 µg) from 9 different cell lines by using anti-FOXA1 monoclonal antibody.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1000

-

Product Specifications

Species

Dog, Human

Expression System

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

OTI3A8

Immunogen

Recombinant protein expressed in E.coli corresponding to amino acids 1-473 of human FOXA1

Conjugate

Unconjugated

Form

lyophilized

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Reconstitute with PBS (pH 7.3) and recommend to perform another round of desalting process using Product # 7KMWCO

Target Information

This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Ci-fkh; fkd7; fkh; Forkhead box protein A1; forkhead-7; FoxA4a; Hepatocyte nuclear factor 3-alpha; HNF-3-alpha; HNF-3A; HNF3-alpha; HNF3alpha; MGC33105; MGC52590; MGC75967; pintallavis; TCF-3A; Transcription factor 3A; xfkh1; xfkh2

Gene Aliases: FOXA1; HNF3A; TCF3A

UniProt ID: (Human) P55317

Entrez Gene ID: (Dog) 490657, (Human) 3169

Molecular Function: DNA binding protein nucleic acid binding transcription factor

negative regulation of transcription from RNA polymerase II promoter chromatin remodeling transcription from RNA polymerase II promoter Notch signaling pathway negative regulation of epithelial to mesenchymal transition covalent chromatin modification dorsal/ventral neural tube patterning response to estradiol positive regulation of intracellular estrogen receptor signaling pathway hormone metabolic process glucose homeostasis positive regulation of neuron differentiation positive regulation of smoothened signaling pathway positive regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter anatomical structure formation involved in morphogenesis neuron fate specification positive regulation of sequence-specific DNA binding transcription factor activity epithelial tube branching involved in lung morphogenesis lung epithelial cell differentiation secretory columnal luminar epithelial cell differentiation involved in prostate glandular acinus development epithelial-mesenchymal signaling involved in prostate gland development prostate gland epithelium morphogenesis prostate gland stromal morphogenesis epithelial cell maturation involved in prostate gland development alveolar secondary septum development dopaminergic neuron differentiation respiratory basal cell differentiation positive regulation of cell-cell adhesion mediated by cadherin

Process(es)

RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding transcription factor binding protein domain specific binding sequence-specific DNA binding transcription regulatory region DNA binding