OriGene

FOXA1 Monoclonal Antibody (OTI3A8), TrueMAB™

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FOXA1 Antibody in Immunocytochemistry (ICC/IF) — Anti-FOXA1 mouse monoclonal antibody (TA500245) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FOXA1(RC206045).

FOXA1 Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOXA1 (RC206045, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-FOXA1. Positive lysates LY401427 (100 µg) and LC401427 (20 µg) can be purchased separately from OriGene.

Product Details

CF500245

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	-

Product Specifications
Species Reactivity	Dog, Human
Host/Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Clone	OTI3A8
Immunogen	Recombinant protein expressed in E.coli corresponding to amino acids 1-473 of human FOXA1 View antigen
Conjugate	Unconjugated
Form	lyophilized
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.3, with 8% trehalose
Contains	no preservative
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Ci-fkh; fkd7; fkh; Forkhead box protein A1; forkhead-7; FoxA4a; Hepatocyte nuclear factor 3-alpha; HNF-3-alpha; HNF-3A; HNF3-alpha; HNF3alpha; MGC33105; MGC52590; MGC75967; pintallavis; TCF-3A; Transcription factor 3A; xfkh1; xfkh2

Gene Aliases: FOXA1; HNF3A; TCF3A

UniProt ID: (Human) P55317

Entrez Gene ID: (Dog) 490657, (Human) 3169

Molecular Function: winged helix/forkhead transcription factor