Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 1
FOXF1 Antibody (MA5-42882) in WB
Western blot analysis of FOXF1 in extracts of various cell lines. Samples were incubated with FOXF1 Monoclonal antibody (Product # MA5-42882) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
MA5-42882
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
8F6G3
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human FOXF1 (Q12946).
Conjugate
Form
Liquid
Concentration
1.07 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2912023
Product Specific Information
Positive test controls include: U2OS, Mouse lung.
Immunogen sequence: MSSAPEKQQP PHGGGGGGGG GGGAAMDPAS SGPSKAKKTN AGIRRPEKPP YSYIALIVMA IQSSPTKRLT LSEIYQFLQS RFPFFRGSYQ GWKNSVRHNL
Target Information
This gene belongs to the forkhead family of transcription factors which is characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in the regulation of pulmonary genes as well as embryonic development. [provided by RefSeq, Jul 2008]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: forkhead box F1a; Forkhead box protein F1; Forkhead, drosophila, homolog-like 5; Forkhead-related activator 1; Forkhead-related protein FKHL5; Forkhead-related transcription factor 1; FREAC-1; Hepatocyte nuclear factor 3 forkhead homolog 8; HFH-8; HNF-3/forkhead homolog 8
Gene Aliases: ACDMPV; AI450827; FKHL5; FOXF1; Foxf1a; Freac-1; FREAC1; HFH-8; Hfh8
UniProt ID: (Human) Q12946, (Mouse) Q61080
Entrez Gene ID: (Human) 2294, (Mouse) 15227
RNA polymerase II regulatory region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
sequence-specific DNA binding
transcription factor activity, sequence-specific DNA binding
transcription regulatory region DNA binding
negative regulation of transcription from RNA polymerase II promoter
blood vessel development
vasculogenesis
in utero embryonic development
somitogenesis
morphogenesis of a branching structure
positive regulation of mesenchymal cell proliferation
endocardial cushion development
cardiac left ventricle morphogenesis
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
smoothened signaling pathway
determination of left/right symmetry
midgut development
mesoderm development
heart development
organ morphogenesis
positive regulation of cell-substrate adhesion
detection of wounding
single organismal cell-cell adhesion
extracellular matrix organization
respiratory tube development
lung development
positive regulation of cell migration
pancreas development
negative regulation of mast cell degranulation
establishment of epithelial cell apical/basal polarity
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
lung alveolus development
lateral mesodermal cell differentiation
embryonic digestive tract morphogenesis
digestive tract development
embryonic digestive tract development
embryonic ectodermal digestive tract morphogenesis
embryonic foregut morphogenesis
negative regulation of inflammatory response
smooth muscle cell differentiation
regulation of smooth muscle cell differentiation
lung morphogenesis
lung vasculature development
trachea development
epithelial tube branching involved in lung morphogenesis
branching involved in open tracheal system development
right lung morphogenesis
lung lobe morphogenesis
venous blood vessel development
epithelial cell differentiation involved in mammary gland alveolus development
cellular response to cytokine stimulus
cellular response to organic cyclic compound
renal system development
ureter development
mesenchyme migration
ductus arteriosus closure
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support