Immunofluorescent analysis of FOXO3 (green) in HeLa cells (left panel) or MCF7 cells (right panel). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a FOXO3 polyclonal antibody (Product # PA1-9042) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.
|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Synthetic peptide corresponding to the C terminus amino acids GAKQASSQSWVPG.|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||1:50 - 1:200|
|Western Blot (WB)||0.1 - 0.3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with bovine, canine, mouse, porcine and rat based on sequence homology.
This antibody was orginally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.
FoxO (forkhead box O) proteins are a family of transcription factors characterized by the distinct forkhead domain. The FoxO proteins act in the insulin signaling pathway, but FoxO3 has also been shown to play a role in the regulation of apoptosis, cell cycle progression and cancer. Several splice variants of FoxO3 have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.