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Product Details
14-9962-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
JC12 (JC-12)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1518792
Product Specific Information
Description: This JC12 monoclonal antibody reacts with human Foxp1, a member of the Forkhead family of winged helix transcription factors. Foxp1 is expressed in nearly every human tissue, including lymphoid (e.g. tonsil, spleen, and thymus), epithelium, kidney, blood, and motor neurons. Altered Foxp1 expression has been observed in, among others, cancers of the breast, lung, colon, prostate, and pancreas. Moreover, patients with diffuse large B cell lymphomas express high levels of Foxp1, which can be utilized as a prognostic marker for survival. Finally, Foxp1 has been reported to be involved in early B cell development (i.e. pro-B cell to pre-B cell transition) and motor neuron specification and connectivity.
Applications Reported: This JC12 antibody has been reported for use in immunoblotting (WB), and immunohistology staining of paraffin embedded tissue sections.
Applications Tested: This JC12 antibody has been tested by western blot analysis on cell lysates prepared from Jurkat cells. This antibody can be used at 1-5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
This gene belongs to subfamily P of the forkhead box (FOX) transcription factor family. Forkhead box transcription factors play important roles in the regulation of tissue- and cell type-specific gene transcription during both development and adulthood. Forkhead box P1 protein contains both DNA-binding- and protein-protein binding-domains. This gene may act as a tumor suppressor as it is lost in several tumor types and maps to a chromosomal region (3p14.1) reported to contain a tumor suppressor gene(s). Alternative splicing results in multiple transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ23741; Fork head related protein like B; fork head-related protein like B; Forkhead box protein P1; Forkhead box protein P1 (FOXP1); Glutamine rich factor 1; glutamine-rich factor 1; Mac-1-regulated forkhead; MFH; MGC12942; MGC88572; MGC99551
Gene Aliases: 12CC4; FOXP1; hFKH1B; HSPC215; MFH; QRF1
UniProt ID: (Human) Q9H334
Entrez Gene ID: (Human) 27086
negative regulation of B cell apoptotic process
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
positive regulation of endothelial cell migration
osteoclast differentiation
response to lipopolysaccharide
regulation of tumor necrosis factor production
chemokine (C-C motif) ligand 2 secretion
osteoclast development
macrophage activation
monocyte activation
endothelial cell activation
regulation of monocyte differentiation
negative regulation of transcription, DNA-templated
positive regulation of smooth muscle cell proliferation
regulation of interleukin-1 beta secretion
regulation of inflammatory response
negative regulation of androgen receptor signaling pathway
T follicular helper cell differentiation
interleukin-21 secretion
regulation of defense response to bacterium
regulation of macrophage colony-stimulating factor production
regulation of endothelial tube morphogenesis
regulation of interleukin-12 secretion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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