Invitrogen

FOXP3 Monoclonal Antibody (236A/E7), eFluor™ 570, eBioscience™

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

29 Published Figures

25 References

View all (142) FOXP3 antibodies

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Datasheet

Protocols

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FOXP3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of formalin-fixed paraffin embedded human tonsil using 5 µg/mL of Mouse IgG1 K Isotype Control eFluor® 570 (left) or 5 µg/mL of Anti-Human Foxp3 eFluor® 570 (right). Nuclei are stained with DAPI.

FOXP3 Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Published figure using FOXP3 monoclonal antibody (Product # 41-4777-82) in Immunohistochemistry (Frozen)

FOXP3 Antibody in Immunohistochemistry (IHC) — Figure 4 Single vs. triple stainings, immunohistochemistry on tonsil sections. ( A - C ) Single stainings: ( A ) Bcl6 red after 15 min TRIS pH 9, ( B ) FoxP3 brown after 20 min TRIS pH 6.1, ( C ) PAX5 blue after 20 min TRIS pH 6.1. ( D ) Human tonsil with triple staining IHC, according to the FOLGAS protocol: Bcl6 red, FoxP3 brown, PAX5 blue; antigen retrievals equivalent to the corresponding single staining. Magnifications indicated by bar. ( E ) Sum of H-scores of the respective stainings under single and triple staining conditions, from two independent observers.

FOXP3 Antibody in Western Blot, Immunoprecipitation (WB, IP) — Published figure using FOXP3 monoclonal antibody (Product # 41-4777-82) in Western Blot and Immunoprecipitation

FOXP3 Antibody in Radioimmune assays (RIA) — Published figure using FOXP3 monoclonal antibody (Product # 41-4777-82) in Radioimmune assays

FOXP3 Antibody — Intracellular staining of human peripheral blood cells. As expected based on known expression patterns, Foxp3 clone 236A/E7 stains CD4+CD25+ T cells and does not stain CD4- cells or CD4+CD25- T cells. Details: Normal human peripheral blood cells were surface stained with CD4 (clone OKT4) and CD25 (clone BC96) followed by intracellular staining with Foxp3 (clone 236A/E7) using the Foxp3/Transcription Factor Staining Buffer Set and protocol. Lymphocytes in the CD4- (left) and CD4+ (right) gates were used for analysis. {RE}

Product Details

41-4777-82

Applications	Tested Dilution	Publications
Immunohistochemistry (Paraffin) (IHC (P))	Assay-Dependent	View 2 publications 2 publications
Immunohistochemistry (Frozen) (IHC (F))	5 µg/mL	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	5 µg/mL	-
Immunohistochemistry (IHC)	-	View 19 publications 19 publications
Radioimmune Assays (RIA)	-	View 1 publication 1 publication
Immunoprecipitation (IP)	-	View 1 publication 1 publication
Western Blot (WB)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Non-human primate, Rhesus monkey
Published species	Not Applicable
Host/Isotype	Mouse / IgG1, kappa
Recommended Isotype Control	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 570, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	236A/E7
Conjugate	eFluor™ 570 Alexa Fluor 488 APC Biotin eFluor 450 eFluor 660 FITC PE PE-Cyanine5 PE-Cyanine7 Unconjugated
Excitation/Emission Max	556/569 nm View spectra
Form	Liquid
Concentration	0.2 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_2573609

Product Specific Information

Description: The 236A/E7 antibody reacts with human Foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of Foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.

Intracellular staining and flow cytometric analysis of freshly isolated human peripheral blood mononuclear cells (PBMCs) with the 236A/E7 antibody using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol reveals staining of the CD4+CD25bright population.

The epitope from 236A/E7 is different from that of PCH101 (cat. 72-5776). This antibody has also been shown to recognize rhesus macaque, sooty mangabey and cynomolgus macaque.

Applications Reported: This 236A/E7 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and microscopy.

Applications Tested: This 236A/E7 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue using high or low pH antigen retrieval and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filter Recommendation: When using this eFluor® 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor® 555 or TRITC filter is acceptable.

Excitation: 555 nm; Emission: 570 nm

Target Information

FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Multiple Immunostainings with Different Epitope Retrievals-The FOLGAS Protocol.

International journal of molecular sciences

von Schoenfeld A,Bronsert P,Poc M,Fuller A,Filby A,Kraft S,Kurowski K,Sörensen K,Huber J,Pfeiffer J,Proietti M,Stehl V,Werner M,Seidl M

Published figure using FOXP3 monoclonal antibody (Product # 41-4777-82) in Immunohistochemistry

Sat Dec 25 00:00:00 EST 2021

Increased CD3⁺, CD8⁺, or FoxP3⁺ T Lymphocyte Infiltrations Are Associated with the Pathogenesis of Colorectal Cancer but Not with the Overall Survival of Patients.

Biology

Barbosa AM,Martinho O,Nogueira R,Campos J,Lobo L,Pinto H,Longatto-Filho A,Castro AG,Martins SF,Torrado E

Published figure using FOXP3 monoclonal antibody (Product # 41-4777-82) in Immunohistochemistry

Fri Aug 20 00:00:00 EDT 2021

Common clonal origin of conventional T cells and induced regulatory T cells in breast cancer patients.

Nature communications

Xydia M,Rahbari R,Ruggiero E,Macaulay I,Tarabichi M,Lohmayer R,Wilkening S,Michels T,Brown D,Vanuytven S,Mastitskaya S,Laidlaw S,Grabe N,Pritsch M,Fronza R,Hexel K,Schmitt S,Müller-Steinhardt M,Halama N,Domschke C,Schmidt M,von Kalle C,Schütz F,Voet T,Beckhove P

Published figure using FOXP3 monoclonal antibody (Product # 41-4777-82) in Immunohistochemistry

Thu Feb 18 00:00:00 EST 2021

Molecular profiling of primary uveal melanomas with tumor-infiltrating lymphocytes.

Oncoimmunology

Triozzi PL,Schoenfield L,Plesec T,Saunthararajah Y,Tubbs RR,Singh AD

Published figure using FOXP3 monoclonal antibody (Product # 41-4777-82) in Immunohistochemistry

Wed Feb 03 00:00:00 EST 2021

Long-lived pancreatic ductal adenocarcinoma slice cultures enable precise study of the immune microenvironment.

Oncoimmunology

Jiang X,Seo YD,Chang JH,Coveler A,Nigjeh EN,Pan S,Jalikis F,Yeung RS,Crispe IN,Pillarisetty VG

Published figure using FOXP3 monoclonal antibody (Product # 41-4777-82) in Immunohistochemistry

Tue Jan 12 00:00:00 EST 2021

Inhibition of RANK signaling in breast cancer induces an anti-tumor immune response orchestrated by CD8+ T cells.

Nature communications

Gómez-Aleza C,Nguyen B,Yoldi G,Ciscar M,Barranco A,Hernández-Jiménez E,Maetens M,Salgado R,Zafeiroglou M,Pellegrini P,Venet D,Garaud S,Trinidad EM,Benítez S,Vuylsteke P,Polastro L,Wildiers H,Simon P,Lindeman G,Larsimont D,Van den Eynden G,Velghe C,Rothé F,Willard-Gallo K,Michiels S,Muñoz P,Walzer T,Planelles L,Penninger J,Azim HA,Loi S,Piccart M,Sotiriou C,González-Suárez E

Published figure using FOXP3 monoclonal antibody (Product # 41-4777-82) in Immunohistochemistry

Thu Dec 10 00:00:00 EST 2020

hESC-derived immune suppressive dendritic cells induce immune tolerance of parental hESC-derived allografts.

EBioMedicine

Todorova D,Zhang Y,Chen Q,Liu J,He J,Fu X,Xu Y

Published figure using FOXP3 monoclonal antibody (Product # 41-4777-82) in Immunohistochemistry

Tue Dec 01 00:00:00 EST 2020

Digital Pathology Analysis Quantifies Spatial Heterogeneity of CD3, CD4, CD8, CD20, and FoxP3 Immune Markers in Triple-Negative Breast Cancer.

Frontiers in physiology

Mi H,Gong C,Sulam J,Fertig EJ,Szalay AS,Jaffee EM,Stearns V,Emens LA,Cimino-Mathews AM,Popel AS

Published figure using FOXP3 monoclonal antibody (Product # 41-4777-82) in Immunohistochemistry

Wed Nov 18 00:00:00 EST 2020

Spontaneous Regression of Ovarian Carcinoma After Septic Peritonitis; A Unique Case Report.

Frontiers in oncology

Roelofsen T,Wefers C,Gorris MAJ,Textor JC,Massuger LFAG,de Vries IJM,van Altena AM

Published figure using FOXP3 monoclonal antibody (Product # 41-4777-82) in Immunohistochemistry

Tue Sep 29 00:00:00 EDT 2020

Distinct Tumor Microenvironments Are a Defining Feature of Strain-Specific CRISPR/Cas9-Induced MPNSTs.

Genes

Scherer A,Stephens VR,McGivney GR,Gutierrez WR,Laverty EA,Knepper-Adrian V,Dodd RD

Published figure using FOXP3 monoclonal antibody (Product # 41-4777-82) in Immunohistochemistry

Sat May 23 00:00:00 EDT 2020

Biomarker recommendation for PD-1/PD-L1 immunotherapy development in pediatric cancer based on digital image analysis of PD-L1 and immune cells.

The journal of pathology. Clinical research

Silva MA,Triltsch N,Leis S,Kanchev I,Tan TH,Van Peel B,Van Kerckhoven M,Deschoolmeester V,Zimmermann J

Published figure using FOXP3 monoclonal antibody (Product # 41-4777-82) in Immunohistochemistry

Wed Apr 01 00:00:00 EDT 2020

Highly multiplexed immunofluorescence images and single-cell data of immune markers in tonsil and lung cancer.

Scientific data

Rashid R,Gaglia G,Chen YA,Lin JR,Du Z,Maliga Z,Schapiro D,Yapp C,Muhlich J,Sokolov A,Sorger P,Santagata S

Published figure using FOXP3 monoclonal antibody (Product # 41-4777-82) in Immunohistochemistry

Tue Dec 17 00:00:00 EST 2019

Multicomponent analysis of the tumour microenvironment reveals low CD8 T cell number, low stromal caveolin-1 and high tenascin-C and their combination as significant prognostic markers in non-small cell lung cancer.

Oncotarget

Onion D,Isherwood M,Shridhar N,Xenophontos M,Craze ML,Day LJ,García-Márquez MA,Pineda RG,Reece-Smith AM,Saunders JH,Duffy JP,Argent RH,Grabowska AM

Published figure using FOXP3 monoclonal antibody (Product # 41-4777-82) in Immunohistochemistry

Fri Jan 05 00:00:00 EST 2018

Preferential accumulation of regulatory T cells with highly immunosuppressive characteristics in breast tumor microenvironment.

Oncotarget

Syed Khaja AS,Toor SM,El Salhat H,Faour I,Ul Haq N,Ali BR,Elkord E

Published figure using FOXP3 monoclonal antibody (Product # 41-4777-82) in Immunohistochemistry

Tue May 16 00:00:00 EDT 2017

Cutting Edge: Selective Oral ROCK2 Inhibitor Reduces Clinical Scores in Patients with Psoriasis Vulgaris and Normalizes Skin Pathology via Concurrent Regulation of IL-17 and IL-10.

Journal of immunology (Baltimore, Md. : 1950)

Zanin-Zhorov A,Weiss JM,Trzeciak A,Chen W,Zhang J,Nyuydzefe MS,Arencibia C,Polimera S,Schueller O,Fuentes-Duculan J,Bonifacio KM,Kunjravia N,Cueto I,Soung J,Fleischmann RM,Kivitz A,Lebwohl M,Nunez M,Woodson J,Smith SL,West RF,Berger M,Krueger JG,Ryan JL,Waksal SD

Published figure using FOXP3 monoclonal antibody (Product # 41-4777-82) in Radioimmune Assays

Mon May 15 00:00:00 EDT 2017

Differential tumor infiltration by T-cells characterizes intrinsic molecular subtypes in breast cancer.

Journal of translational medicine

Miyan M,Schmidt-Mende J,Kiessling R,Poschke I,de Boniface J

Published figure using FOXP3 monoclonal antibody (Product # 41-4777-82) in Immunohistochemistry (Frozen)

Fri Jul 29 00:00:00 EDT 2016

Regulatory T Cell Modulation by CBP/EP300 Bromodomain Inhibition.

The Journal of biological chemistry

Ghosh S,Taylor A,Chin M,Huang HR,Conery AR,Mertz JA,Salmeron A,Dakle PJ,Mele D,Cote A,Jayaram H,Setser JW,Poy F,Hatzivassiliou G,DeAlmeida-Nagata D,Sandy P,Hatton C,Romero FA,Chiang E,Reimer T,Crawford T,Pardo E,Watson VG,Tsui V,Cochran AG,Zawadzke L,Harmange JC,Audia JE,Bryant BM,Cummings RT,Magnuson SR,Grogan JL,Bellon SF,Albrecht BK,Sims RJ,Lora JM

Published figure using FOXP3 monoclonal antibody (Product # 41-4777-82) in Immunoprecipitation

Fri Jun 17 00:00:00 EDT 2016

Regulatory T Cell Modulation by CBP/EP300 Bromodomain Inhibition.

The Journal of biological chemistry

Published figure using FOXP3 monoclonal antibody (Product # 41-4777-82) in Immunoprecipitation

Fri Jun 17 00:00:00 EDT 2016

The immune microenvironment of breast ductal carcinoma in situ.

Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc

Thompson E,Taube JM,Elwood H,Sharma R,Meeker A,Warzecha HN,Argani P,Cimino-Mathews A,Emens LA

Published figure using FOXP3 monoclonal antibody (Product # 41-4777-82) in Immunohistochemistry

Tue Mar 01 00:00:00 EST 2016

Follicular regulatory T cells impair follicular T helper cells in HIV and SIV infection.

Nature communications

Miles B,Miller SM,Folkvord JM,Kimball A,Chamanian M,Meditz AL,Arends T,McCarter MD,Levy DN,Rakasz EG,Skinner PJ,Connick E

Published figure using FOXP3 monoclonal antibody (Product # 41-4777-82) in Immunofluorescence

Tue Oct 20 00:00:00 EDT 2015

Altered expression of autoimmune regulator in infant down syndrome thymus, a possible contributor to an autoimmune phenotype.

Journal of immunology (Baltimore, Md. : 1950)

Skogberg G,Lundberg V,Lindgren S,Gudmundsdottir J,Sandström K,Kämpe O,Annerén G,Gustafsson J,Sunnegårdh J,van der Post S,Telemo E,Berglund M,Ekwall O

Published figure using FOXP3 monoclonal antibody (Product # 41-4777-82) in Immunohistochemistry

Mon Sep 01 00:00:00 EDT 2014

IDO and regulatory T cell support are critical for cytotoxic T lymphocyte-associated Ag-4 Ig-mediated long-term solid organ allograft survival.

Journal of immunology (Baltimore, Md. : 1950)

Sucher R,Fischler K,Oberhuber R,Kronberger I,Margreiter C,Ollinger R,Schneeberger S,Fuchs D,Werner ER,Watschinger K,Zelger B,Tellides G,Pilat N,Pratschke J,Margreiter R,Wekerle T,Brandacher G

Published figure using FOXP3 monoclonal antibody (Product # 41-4777-82) in Immunohistochemistry

Sun Jan 01 00:00:00 EST 2012

CD4(+) regulatory T cells in a cynomolgus macaque model of Mycobacterium tuberculosis infection.

The Journal of infectious diseases

Green AM,Mattila JT,Bigbee CL,Bongers KS,Lin PL,Flynn JL

Sun Aug 15 00:00:00 EDT 2010

Gamma c-signaling cytokines induce a regulatory T cell phenotype in malignant CD4+ T lymphocytes.

Journal of immunology (Baltimore, Md. : 1950)

Kasprzycka M,Zhang Q,Witkiewicz A,Marzec M,Potoczek M,Liu X,Wang HY,Milone M,Basu S,Mauger J,Choi JK,Abrams JT,Hou JS,Rook AH,Vonderheid E,Woetmann A,Odum N,Wasik MA

Published figure using FOXP3 monoclonal antibody (Product # 41-4777-82) in Immunohistochemistry

Fri Aug 15 00:00:00 EDT 2008

Early resolution of acute immune activation and induction of PD-1 in SIV-infected sooty mangabeys distinguishes nonpathogenic from pathogenic infection in rhesus macaques.

Journal of immunology (Baltimore, Md. : 1950)

Estes JD,Gordon SN,Zeng M,Chahroudi AM,Dunham RM,Staprans SI,Reilly CS,Silvestri G,Haase AT

Thu May 15 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: Forkhead box protein P3; Forkhead box protein P3 41 kDa form; Forkhead box protein P3, C-terminally processed; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; MGC141961; MGC141963; regulatory protein Foxp3; RP23-54C14.1; Scurfin

Gene Aliases: AIID; DIETER; FOXP3; IPEX; JM2; PIDX; XPID

UniProt ID: (Human) Q9BZS1

Entrez Gene ID: (Human) 50943, (Rhesus monkey) 574303

Function(s)

Process(es)

RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription corepressor activity protein binding histone acetyltransferase binding protein homodimerization activity histone deacetylase binding sequence-specific DNA binding metal ion binding NF-kappaB binding NFAT protein binding

FOXP3 Monoclonal Antibody (236A/E7), eFluor™ 570, eBioscience™

Product Details

41-4777-82

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Immunohistochemistry (IHC)

Radioimmune Assays (RIA)

Immunoprecipitation (IP)

Western Blot (WB)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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