Invitrogen

FOXP3 Monoclonal Antibody (236A/E7), eFluor™ 450, eBioscience™

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

10 References

View all (163) FOXP3 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (1)
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FOXP3 Antibody (48-4777-42) in Flow

Surface staining of normal human peripheral blood cells with Anti-Human CD25 APC (Product # 17-0259-42) followed by fixation and permeabilization with the Foxp3/Transcription Factor Staining Buffers (Product # 00-5523-00) and intracellular staining with Mouse IgG1 K Isotype Control eFluor® 450 (Product # 48-4714-82) (left) or Anti-Human Foxp3 eFluor® 450 (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

FOXP3 Antibody — Intracellular staining of human peripheral blood cells. As expected based on known expression patterns, Foxp3 clone 236A/E7 stains CD4+CD25+ T cells and does not stain CD4- cells or CD4+CD25- T cells. Details: Normal human peripheral blood cells were surface stained with CD4 (clone OKT4) and CD25 (clone BC96) followed by intracellular staining with Foxp3 (clone 236A/E7) using the Foxp3/Transcription Factor Staining Buffer Set and protocol. Lymphocytes in the CD4- (left) and CD4+ (right) gates were used for analysis. {RE}

Product Details

48-4777-42

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 9 publications 9 publications

Product Specifications

Species Reactivity

Human, Non-human primate, Rhesus monkey

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 450, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

236A/E7

Conjugate

eFluor™ 450 eFluor™ 450 eFluor™ 450

Excitation/Emission Max

405/445 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1548676

Product Specific Information

Description: The 236A/E7 antibody reacts with human foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.

Intracellular staining and flow cytometric analysis of freshly isolated human peripheral blood mononuclear cells (PBMCs) with the 236A/E7 antibody using the Foxp3 Staining Buffer Set (Product # 00-5523) and protocol reveals staining of the CD4+CD25bright population.

The epitope from 236A/E7 is different from that of PCH101 (Product # 72-5776-40).

Applications Reported: This 236A/E7 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 236A/E7 antibody has been pre-titrated and tested using the Foxp3/Transctiption Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Vaccine plus microbicide effective in preventing vaginal SIV transmission in macaques.

Nature microbiology

Rahman MA,Bissa M,Silva de Castro I,Helmold Hait S,Stamos JD,Bhuyan F,Hunegnaw R,Sarkis S,Gutowska A,Doster MN,Moles R,Hoang T,Miller Jenkins LM,Appella E,Venzon DJ,Choo-Wosoba H,Cardozo T,Baum MM,Appella DH,Robert-Guroff M,Franchini G

48-4777-42 was used in Flow cytometry/Cell sorting to show that, by increasing zinc availability, SAMT-247 increases natural killer cytotoxicity and monocyte efferocytosis, and decreases T-cell activation to augment vaccine-induced protection.

Mon May 01 00:00:00 EDT 2023

Increased circulating regulatory T cells in medicated people with schizophrenia.

Psychiatry research

Kelly DL,Li X,Kilday C,Feldman S,Clark S,Liu F,Buchanan RW,Tonelli LH

48-4777 was used in Flow cytometry/Cell sorting to suggest that Tregs may contribute to improved negative symptoms in schizophrenia, possibly by counteracting on-going inflammatory processes.

Thu Nov 01 00:00:00 EDT 2018

Lactobacillus rhamnosus GG-Derived Soluble Mediators Modulate Adaptive Immune Cells.

Frontiers in immunology

Ludwig IS,Broere F,Manurung S,Lambers TT,van der Zee R,van Eden W

48-4777 was used in Flow cytometry/Cell sorting to determine the role of Lactobacillus rhamosus soluble mediators in adaptive immune responses.

Tue Sep 26 00:00:00 EDT 2023

Identifying functional defects in patients with immune dysregulation due to LRBA and CTLA-4 mutations.

Blood

Hou TZ,Verma N,Wanders J,Kennedy A,Soskic B,Janman D,Halliday N,Rowshanravan B,Worth A,Qasim W,Baxendale H,Stauss H,Seneviratne S,Neth O,Olbrich P,Hambleton S,Arkwright PD,Burns SO,Walker LS,Sansom DM

48-4777 was used in Flow cytometry/Cell sorting to develop assays capable of distinguishing a variety of defects in the CTLA-4 pathway.

Thu Mar 16 00:00:00 EDT 2017

A novel method for assaying human regulatory T cell direct suppression of B cell effector function.

Journal of immunological methods

Weingartner E,Courneya JP,Keegan A,Golding A

48477742 was used in flow cytometry to develop a method to assess the capacity of human regulatory T cells to suppress IgM production from an immature human B cell line

Wed Feb 01 00:00:00 EST 2017

Selective Sparing of Human Tregs by Pharmacologic Inhibitors of the Phosphatidylinositol 3-Kinase and MEK Pathways.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

Zwang NA,Zhang R,Germana S,Fan MY,Hastings WD,Cao A,Turka LA

48477742 was used in flow cytometry to study the pharmacologic inhibition of PI3K and MEK pathways in mixed cultures of human mononuclear cells.

Thu Sep 01 00:00:00 EDT 2016

TGF-β Affects the Differentiation of Human GM-CSF⁺ CD4⁺ T Cells in an Activation- and Sodium-Dependent Manner.

Frontiers in immunology

Éliás S,Schmidt A,Kannan V,Andersson J,Tegnér J

48-4777 was used in Flow cytometry/Cell sorting to study the environmental cues that promote differentiation of granulocyte-macrophage colony-stimulating factor producing T cells.

Wed Nov 20 00:00:00 EST 2019

A fully human IgG1 anti-PD-L1 MAb in an in vitro assay enhances antigen-specific T-cell responses.

Clinical & translational immunology

Grenga I,Donahue RN,Lepone LM,Richards J,Schlom J

48-4777 was used in Flow cytometry/Cell sorting to explore the effects of Avelumab: a checkpoint-blocking mAB targeting PD-L1, on the immune responses in antigen-specific IVS assays.

Sun May 01 00:00:00 EDT 2016

Hypomethylation at the regulatory T cell-specific demethylated region in CD25hi T cells is decoupled from FOXP3 expression at the inflamed site in childhood arthritis.

Journal of immunology (Baltimore, Md. : 1950)

Bending D,Pesenacker AM,Ursu S,Wu Q,Lom H,Thirugnanabalan B,Wedderburn LR

48-4777 was used in Flow cytometry/Cell sorting to study dissociation of CD25 and FOXP3 in CD4+ T cells at the site of inflammation in patients with juvenile arthritis..

Mon Sep 15 00:00:00 EDT 2014

Early resolution of acute immune activation and induction of PD-1 in SIV-infected sooty mangabeys distinguishes nonpathogenic from pathogenic infection in rhesus macaques.

Journal of immunology (Baltimore, Md. : 1950)

Estes JD,Gordon SN,Zeng M,Chahroudi AM,Dunham RM,Staprans SI,Reilly CS,Silvestri G,Haase AT

Thu May 15 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: Forkhead box protein P3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; MGC141961; MGC141963; regulatory protein Foxp3; RP23-54C14.1; Scurfin

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Gene Aliases: AIID; DIETER; FOXP3; IPEX; JM2; PIDX; XPID

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UniProt ID: (Human) Q9BZS1

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Entrez Gene ID: (Human) 50943, (Rhesus monkey) 574303

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Function(s)

RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription corepressor activity protein binding histone acetyltransferase binding protein homodimerization activity histone deacetylase binding sequence-specific DNA binding metal ion binding NF-kappaB binding NFAT protein binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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FOXP3 Monoclonal Antibody (236A/E7), eFluor™ 450, eBioscience™

FOXP3 Antibody (48-4777-42) in Flow

Product Details

48-4777-42

Immunohistochemistry (Paraffin) (IHC (P))

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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