Invitrogen

FOXP3 Monoclonal Antibody (NRRF-30), PE, eBioscience™

FIGURE: 1 / 1

FOXP3 Antibody (12-4771-82) in Flow

BALB/c splenocytes were surface-stained with Anti-Mouse CD4 FITC (Product # 11-0042-82) (left) and Anti-Mouse CD25 APC (Product # 17-0251-82) (right), then intracellularly with Anti-Mouse Foxp3 PE using the Foxp3 Staining Buffer Set (Product # 00-5523-00). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

Product Details

12-4771-82

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.25 µg/test

View 39 publications 39 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

NRRF-30

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_529580

Product Specific Information

Description: The NRRF-30 antibody reacts with mouse Foxp3 also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of foxP3 mRNA has been shown in CD4+/CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+/CD25- cells imparts a Treg phenotype in these cells.

Immunoblotting with NRRF-30 antibody has mapped the epitope to amino acids 1-75 of the mouse Foxp3 protein.

Intracellular staining of mouse splenocytes with fluorochrome-conjugated NRRF-30 using the eBioscience Foxp3 Staining Buffers () and corresponding staining protocol reveals approximately 3% of total cells in the C57Bl/6 strain and approximately 5% in the BALB/c mouse strain. Multicolor flow cytometric analysis demonstrates approximately 90% of the CD4+/CD2+ cells and 4% of the CD4+/CD25- cells staining with NRRF-30. Co-staining with FJK-16s (anti-mouse/rat Foxp3, Product # 71-5775-40), which has been mapped to amino acids 71-125, and NRRF-30 shows 100% correlation, indicating that the same cells are stained with both anti-mouse Foxp3 antibodies.

Please see our FAQ regarding the usage of eBioscience Foxp3 reagents.

Applications Reported: This NRRF-30 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This NRRF-30 antibody has been tested by intracellular flow cytometric analysis of mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please see Best Protocols Section (Staining Intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Identification of cell types in multiplexed in situ images by combining protein expression and spatial information using CELESTA.

Nature methods

Zhang W,Li I,Reticker-Flynn NE,Good Z,Chang S,Samusik N,Saumyaa S,Li Y,Zhou X,Liang R,Kong CS,Le QT,Gentles AJ,Sunwoo JB,Nolan GP,Engleman EG,Plevritis SK

12-4771-82 was used in In vivo experiments to demonstrate the power of CELESTA to facilitate identification of clinically relevant interactions.

Wed Jun 01 00:00:00 EDT 2022

Obesity results in adipose tissue T cell exhaustion.

JCI insight

Porsche CE,Delproposto JB,Geletka L,O'Rourke R,Lumeng CN

12-4771-82 was used in Flow Cytometry to assess adipose tissue T cells activation and inflammatory potential within mouse and human stromal vascular fraction.

Thu Apr 22 00:00:00 EDT 2021

Activin A Promotes Regulatory T-cell-Mediated Immunosuppression in Irradiated Breast Cancer.

Cancer immunology research

De Martino M,Daviaud C,Diamond JM,Kraynak J,Alard A,Formenti SC,Miller LD,Demaria S,Vanpouille-Box C

12-4771-82 was used in Flow Cytometry to shed light on an immune escape mechanism driven by activin A and suggest that dual targeting of activin A and TGFβ may be required to optimally unleash radiation-induced antitumor immunity against breast cancer.

Fri Jan 01 00:00:00 EST 2021

Ding's herbal enema treats dextran sulfate sodium-induced colitis in mice by regulating the gut microbiota and maintaining the Treg/Th17 cell balance.

Experimental and therapeutic medicine

Tan YY,Ding Y,Zheng X,Dai GJ,Zhang SM,Yang X,Xu DC,Chen P,Zhang JM,Ma JZ,Li M,Huang SC,Liu Y,Zhang YT,Xing H,Ding K,Ding YJ

12-4771-82 was used in Flow cytometry/Cell sorting to determine the molecular mechanism of the effect of DHEP in UC treatment.

Wed Dec 01 00:00:00 EST 2021

Adipose tissue dendritic cell signals are required to maintain T cell homeostasis and obesity-induced expansion.

Molecular and cellular endocrinology

Porsche CE,Delproposto JB,Patrick E,Zamarron BF,Lumeng CN

12-4771 was used in Flow cytometry/Cell sorting to investigate the contribution of dendritic cells to adipose tissue derived chronic inflammation as professional antigen presenting cells in adipose issue.

Sun Apr 05 00:00:00 EDT 2020

Bacteria-triggered tumor-specific thrombosis to enable potent photothermal immunotherapy of cancer.

Science advances

Yi X,Zhou H,Chao Y,Xiong S,Zhong J,Chai Z,Yang K,Liu Z

12-4771 was used in Flow Cytometry/Cell Sorting to demonstrate that bacteria by themselves could act as a tumor-specific PTT agent to enable photoimmunotherapy cancer therapy to inhibit tumor metastasis and recurrence.

Sat Aug 01 00:00:00 EDT 2020

Artificial Mini Dendritic Cells Boost T Cell-Based Immunotherapy for Ovarian Cancer.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Cheng S,Xu C,Jin Y,Li Y,Zhong C,Ma J,Yang J,Zhang N,Li Y,Wang C,Yang Z,Wang Y

12-4771-82 was used in Flow Cytometry to suggest that mini dendritic cell (DC) may serve as a facile and potent vaccine to boost anticancer immunotherapy.

Wed Apr 01 00:00:00 EDT 2020

ICOS agonism by JTX-2011 (vopratelimab) requires initial T cell priming and Fc cross-linking for optimal T cell activation and anti-tumor immunity in preclinical models.

PloS one

Hanson A,Elpek K,Duong E,Shallberg L,Fan M,Johnson C,Wallace M,Mabry GR,Sazinsky S,Pepper L,Shu CJ,Sathyanarayanan S,Zuerndorfer S,Simpson T,Gostissa M,Briskin M,Law D,Michaelson J,Harvey CJ

12-4771 was used in Flow cytometry/Cell sorting to describe the generation of specific, species cross-reactive, agonist antibodies to ICOS, including the humanized clinical candidate, JTX-2011 (vopratelimab).

Mon Nov 16 00:00:00 EST 2020

Mesenchymal Stem and Stromal Cells Harness Macrophage-Derived Amphiregulin to Maintain Tissue Homeostasis.

Cell reports

Ko JH,Kim HJ,Jeong HJ,Lee HJ,Oh JY

12-4771-82 was used in Flow Cytometry to demonstrate the role of mesenchymal stem and stromal cell and macrophage cross-talk in post-injury tissue homeostasis in mice.

Tue Mar 17 00:00:00 EDT 2020

IFN-γ promoted exosomes from mesenchymal stem cells to attenuate colitis via miR-125a and miR-125b.

Cell death & disease

Yang R,Huang H,Cui S,Zhou Y,Zhang T,Zhou Y

12-4771-82 was used in Flow Cytometry to investigate the potential anti-inflammatory effects of bone marrow mesenchymal stem cell-derived exosomes.

Thu Jul 30 00:00:00 EDT 2020

Enhanced Therapeutic Efficacy of a Novel Oncolytic Herpes Simplex Virus Type 2 Encoding an Antibody Against Programmed Cell Death 1.

Molecular therapy oncolytics

Zhu Y,Hu X,Feng L,Yang Z,Zhou L,Duan X,Cheng S,Zhang W,Liu B,Zhang K

12-4771-82 was used in Flow Cytometry to develop a novel oncolytic herpes simplex virus 2 (HSV2) encoding an anti-human programmed cell death 1 (PD-1) monoclonal antibody (oHSV2-aPD1) and demostrate its therapeutic efficacy.

Fri Dec 20 00:00:00 EST 2019

Bilateral Effect of the Unilateral Corneal Nerve Cut on Both Ocular Surface and Lacrimal Gland.

Investigative ophthalmology & visual science

Lee HK,Kim KW,Ryu JS,Jeong HJ,Lee SM,Kim MK

12-4771 was used in Flow cytometry/Cell sorting to demonstrate that a unilateral cut of the corneal nerve results in activation of immune cells on the ocular surface and dysregulation of lacrimal secretion bilaterally through the bidirectional neuronal signals.

Wed Jan 02 00:00:00 EST 2019

Checkpoint blockade and nanosonosensitizer-augmented noninvasive sonodynamic therapy combination reduces tumour growth and metastases in mice.

Nature communications

Yue W,Chen L,Yu L,Zhou B,Yin H,Ren W,Liu C,Guo L,Zhang Y,Sun L,Zhang K,Xu H,Chen Y

12-4771 was used in Flow cytometry/Cell sorting to demonstrate how a combination of nanosonosensitiser-augmented sonodynamic and anti-PD-L1 checkpoint blockade therapy deliver highly efficient tumour therapy in mice.

Thu May 02 00:00:00 EDT 2019

Intraperitoneal neutrophils activated by KRAS-induced ovarian cancer exert antitumor effects by modulating adaptive immunity.

International journal of oncology

Yoshida M,Taguchi A,Kawana K,Ogishima J,Adachi K,Kawata A,Nakamura H,Sato M,Fujimoto A,Inoue T,Tomio K,Mori M,Nagamatsu T,Arimoto T,Koga K,Hiraike OW,Oda K,Kiyono T,Osuga Y,Fujii T

Mon Oct 01 00:00:00 EDT 2018

A highly potent and selective inhibitor Roxyl-WL targeting IDO1 promotes immune response against melanoma.

Journal of enzyme inhibition and medicinal chemistry

Xu G,Wang T,Li Y,Huang Z,Wang X,Zheng J,Yang S,Fan Y,Xiang R

12-4771 was used in Flow cytometry/Cell sorting to discover a novel inhibitor of indoleamine 2,3-dioxygenase 1 with an increased potent activity compared with other inhibitors in clinical trials.

Sat Dec 01 00:00:00 EST 2018

Loss of Gαq impairs regulatory B-cell function.

Arthritis research & therapy

He Y,Yuan X,Li Y,Zhong C,Liu Y,Qian H,Xuan J,Duan L,Shi G

12-4771 was used in Flow cytometry/Cell sorting to study the role of Gaq in the modulation of regulatory B cell function.

Fri Aug 24 00:00:00 EDT 2018

Protective function of Bu Shen Huo Xue formula on the immunity of B6AF1 mice with experimental autoimmune premature ovarian failure.

Experimental and therapeutic medicine

Wang P,Lu Y,Chen S,Chen Y,Hu C,Zuo Y

12-4771-82 was used in Flow Cytometry to evaluate the immunoprotective effects of Bu Shen Huo Xue formula on premature ovarian failure and the underlying mechanisms.

Sun Apr 01 00:00:00 EDT 2018

A cancer vaccine-mediated postoperative immunotherapy for recurrent and metastatic tumors.

Nature communications

Wang T,Wang D,Yu H,Feng B,Zhou F,Zhang H,Zhou L,Jiao S,Li Y

12-4771 was used in Flow cytometry/Cell sorting to assess the efficacy of a personalised cancer vaccine for eliciting postsurgical tumour specific-immunity.

Wed Apr 18 00:00:00 EDT 2018

The Combined Use of Melatonin and an Indoleamine 2,3-Dioxygenase-1 Inhibitor Enhances Vaccine-Induced Protective Cellular Immunity to HPV16-Associated Tumors.

Frontiers in immunology

Moreno ACR,Porchia BFMM,Pagni RL,Souza PDC,Pegoraro R,Rodrigues KB,Barros TB,Aps LRMM,de Araújo EF,Calich VLG,Ferreira LCS

Thu Sep 19 00:00:00 EDT 2019

Antibody Tumor Targeting Is Enhanced by CD27 Agonists through Myeloid Recruitment.

Cancer cell

Turaj AH,Hussain K,Cox KL,Rose-Zerilli MJJ,Testa J,Dahal LN,Chan HTC,James S,Field VL,Carter MJ,Kim HJ,West JJ,Thomas LJ,He LZ,Keler T,Johnson PWM,Al-Shamkhani A,Thirdborough SM,Beers SA,Cragg MS,Glennie MJ,Lim SH

12-4771 was used in Flow cytometry/Cell sorting to demonstrate the therapeutic advantage of using an immunomodulatory mAb to regulate lymphoid cells, which then recruit and activate myeloid cells for enhanced killing of mAb-opsonized tumors.

Mon Dec 11 00:00:00 EST 2017

Herpes Simplex Virus Glycoprotein D Targets a Specific Dendritic Cell Subset and Improves the Performance of Vaccines to Human Papillomavirus-Associated Tumors.

Molecular cancer therapeutics

Porchia BFMM,Moreno ACR,Ramos RN,Diniz MO,de Andrade LHTM,Rosa DS,Barbuto JAM,Boscardin SB,Ferreira LCS

Fri Sep 01 00:00:00 EDT 2017

Respiratory Syncytial Virus Exacerbates OVA-mediated asthma in mice through C5a-C5aR regulating CD4⁺T cells Immune Responses.

Scientific reports

Hu X,Li X,Hu C,Qin L,He R,Luo L,Tang W,Feng J

12-4771 was used in Flow cytometry/Cell sorting to indicate that respiratory syncytial virus infection can increase C5a and C5aR expression in the pathogenesis of infected, asthmatic mice.

Thu Nov 09 00:00:00 EST 2017

Collagen epitope expression on B cells is sufficient to confer tolerance to collagen-induced arthritis.

Arthritis research & therapy

Andersson SE,Eneljung T,Tengvall S,Jirholt P,Stern A,Henningsson L,Liang B,Thorarinsdottir K,Kihlberg J,Holmdahl R,Mårtensson IL,Gustafsson K,Gjertsson I

12-4771 was used in Flow cytometry/Cell sorting to investigate how endogenous presentation of the CII-peptide on B cells is one of the key contributors to arthritis tolerance induction and maintenance.

Tue Jun 14 00:00:00 EDT 2016

Hydrogel dual delivered celecoxib and anti-PD-1 synergistically improve antitumor immunity.

Oncoimmunology

Li Y,Fang M,Zhang J,Wang J,Song Y,Shi J,Li W,Wu G,Ren J,Wang Z,Zou W,Wang L

12-4771 was used in Flow cytometry/Cell sorting to assess an alginate-hydrogel-mediated, combinatorial therapy of celecoxib and PD-1 mAb as a potential valuable regimen for treating human cancer.

Mon Feb 01 00:00:00 EST 2016

Gene Therapy Induces Antigen-Specific Tolerance in Experimental Collagen-Induced Arthritis.

PloS one

Tengvall S,Eneljung T,Jirholt P,Turesson O,Wing K,Holmdahl R,Kihlberg J,Stern A,Mårtensson IL,Henningsson L,Gustafsson K,Gjertsson I

12-4771 was used in Flow cytometry/Cell sorting to investigate induction of immunological tolerance by lentiviral based gene therapy in a mouse model of rheumatoid arthritis.

Mon Jul 17 00:00:00 EDT 2017

Schistosoma mansoni Tegument (Smteg) Induces IL-10 and Modulates Experimental Airway Inflammation.

PloS one

Marinho FV,Alves CC,de Souza SC,da Silva CM,Cassali GD,Oliveira SC,Pacifico LG,Fonseca CT

12-4771 was used in Flow cytometry/Cell sorting to analyse airway inflammation in asthmatic mice challenged with ovalbumin after intraperitoneal inoculation with Smteg.

Mon Jul 24 00:00:00 EDT 2017

Virulence-Dependent Alterations in the Kinetics of Immune Cells during Pulmonary Infection by Mycobacterium tuberculosis.

PloS one

Kim WS,Kim JS,Cha SB,Han SJ,Kim H,Kwon KW,Kim SJ,Eum SY,Cho SN,Shin SJ

12-4771 was used in Flow cytometry/Cell sorting to compare the bacterial burden, inflammation and kinetics of immune cells during aerogenic infection in the lung between laboratory-adapted strains (Mtb H37Rv and H37Ra) and Mtb K strain.

Mon Jun 27 00:00:00 EDT 2016

Ubiquitous Over-Expression of Chromatin Remodeling Factor SRG3 Ameliorates the T Cell-Mediated Exacerbation of EAE by Modulating the Phenotypes of both Dendritic Cells and Macrophages.

PloS one

Lee SW,Park HJ,Jeon SH,Lee C,Seong RH,Park SH,Hong S

12-4771 was used in Flow cytometry/Cell sorting to investigate the role of a SWI3-related gene in experimental autoimmune encephalomyelitis development.

Mon Apr 11 00:00:00 EDT 2016

Low-dose 5-aza-2'-deoxycytidine pretreatment inhibits experimental autoimmune encephalomyelitis by induction of regulatory T cells.

Molecular medicine (Cambridge, Mass.)

Chan MW,Chang CB,Tung CH,Sun J,Suen JL,Wu SF

12-4771 was used in Flow cytometry/Cell sorting to investigate whether loss of P53 function contributes to persistent activation of STAT3 and modification of the pancreatic tumor stroma.

Thu Jun 26 00:00:00 EDT 2014

Foxp3 expression in macrophages associated with RENCA tumors in mice.

PloS one

Devaud C,Yong CS,John LB,Westwood JA,Duong CP,House CM,Denoyer D,Li J,Darcy PK,Kershaw MH

12-4771 was used in Flow cytometry/Cell sorting to study how tumour-associated macrophages could express Foxp3.

Mon Nov 16 00:00:00 EST 2015

Autoimmune diabetes is suppressed by treatment with recombinant human tissue Kallikrein-1.

PloS one

Maneva-Radicheva L,Amatya C,Parker C,Ellefson J,Radichev I,Raghavan A,Charles ML,Williams MS,Robbins MS,Savinov AY

12-4771 was used in Flow cytometry/Cell sorting to reveal that the protein, DM199, may benefit type 1 diabetics by attenuating autoimmunity and improving beta cell health.

Wed Jun 10 00:00:00 EDT 2015

Killer Treg cells ameliorate inflammatory insulitis in non-obese diabetic mice through local and systemic immunomodulation.

International immunology

Kaminitz A,Yolcu ES,Mizrahi K,Shirwan H,Askenasy N

12-4771 was used in Flow cytometry/Cell sorting to evaluate the capacity of naive and killer Treg cells to modulate the course of inflammatory insulitis after onset of hyperglycemia in NOD mice.

Thu Aug 01 00:00:00 EDT 2013

R-phycoerythrin-conjugated antibodies are inappropriate for intracellular staining of murine plasma cells.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Kim MS,Kim TS

12-4771-82 was used in Flow cytometry/Cell sorting to show that PE-conjugated antibodies are inappropriate for intracellular staining of murine plasma cells.

Wed May 01 00:00:00 EDT 2013

Ikaros sets the potential for Th17 lineage gene expression through effects on chromatin state in early T cell development.

The Journal of biological chemistry

Wong LY,Hatfield JK,Brown MA

12-4771 was used in Flow cytometry/Cell sorting to investigate the factors that influence the development of Th17 cells, showing that lineage gene expression is set by Ikaros.

Fri Dec 06 00:00:00 EST 2013

Antigen-specific gene therapy after immunisation reduces the severity of collagen-induced arthritis.

Clinical & developmental immunology

Eneljung T,Tengvall S,Jirholt P,Henningsson L,Holmdahl R,Gustafsson K,Gjertsson I

12-4771 was used in Flow cytometry/Cell sorting to show antigen-specific gene therapy is effective when administered during the inflammatory course of arthritis.

Mon Aug 04 00:00:00 EDT 2014

Memory phenotype CD4 T cells undergoing rapid, nonburst-like, cytokine-driven proliferation can be distinguished from antigen-experienced memory cells.

PLoS biology

Younes SA,Punkosdy G,Caucheteux S,Chen T,Grossman Z,Paul WE

12-4771 was used in Flow cytometry/Cell sorting to identify and characterise two sub-populations of memory T cells.

Sat Oct 01 00:00:00 EDT 2011

Chronic chemoimmunotherapy achieves cure of spontaneous murine mammary tumors via persistent blockade of posttherapy counter-regulation.

Journal of immunology (Baltimore, Md. : 1950)

Rowswell-Turner RB,Harden JL,Nair RE,Gu T,Kilinc MO,Egilmez NK

12-4771 was used in Flow cytometry/Cell sorting to demonstrate that chronic administration of cyclophosphamide in conjunction with immunotherapy sustains the cytotoxic activity of antitumor T-effector cells.

Sat Oct 15 00:00:00 EDT 2011

Effector and naturally occurring regulatory T cells display no abnormalities in activation induced cell death in NOD mice.

PloS one

Kaminitz A,Yolcu ES,Askenasy EM,Stein J,Yaniv I,Shirwan H,Askenasy N

12-4771 was used in Flow cytometry/Cell sorting to document negative regulation of effector and suppressor cells by Fas cross-linking and dissociation between sensitivity to apoptosis and proliferation in stimulated Treg.

Thu Dec 01 00:00:00 EST 2011

Regulatory B cells prevent and reverse allergic airway inflammation via FoxP3-positive T regulatory cells in a murine model.

The Journal of allergy and clinical immunology

Amu S,Saunders SP,Kronenberg M,Mangan NE,Atzberger A,Fallon PG

12-4771 was used in Immunocytochemistry to identify and characterise the specific helminth-induced regulatory B-cell subpopulation, and determine the mechanism by which these regulatory B cells suppress allergic airway inflammation.

Sat May 01 00:00:00 EDT 2010

Tyramide signal amplification for analysis of kinase activity by intracellular flow cytometry.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Clutter MR,Heffner GC,Krutzik PO,Sachen KL,Nolan GP

12-4771 was used in Flow cytometry/Cell sorting to optimise the tyramide signal amplification method to profile Stat1 phosphorylation in primary immune system cells.

Mon Nov 01 00:00:00 EDT 2010

Apoptosis of purified CD4+ T cell subsets is dominated by cytokine deprivation and absence of other cells in new onset diabetic NOD mice.

PloS one

Kaminitz A,Askenasy EM,Yaniv I,Stein J,Askenasy N

12-4771 was used in Flow cytometry/Cell sorting to study the sensitivity of Tregs to apoptosis in diabetic NOD mice.

Fri Dec 31 00:00:00 EST 2010

Bioinformatics

Protein Aliases: Forkhead box protein P3; MGC141961; MGC141963; RP23-54C14.1; Scurfin; scurfy

View more View less

Gene Aliases: Foxp3; JM2; scurfin; sf

View more View less

UniProt ID: (Mouse) Q99JB6

View more View less

Entrez Gene ID: (Mouse) 20371

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Function(s)

core promoter binding nucleic acid binding DNA binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription corepressor activity protein binding histone acetyltransferase binding protein homodimerization activity histone deacetylase binding sequence-specific DNA binding metal ion binding NFAT protein binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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FOXP3 Monoclonal Antibody (NRRF-30), PE, eBioscience™

FOXP3 Antibody (12-4771-82) in Flow

Product Details

12-4771-82

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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