Spectral confocal microscopy of CHO cells using MA1-17769. CHO cells were transiently transfected with an expression vector encoding FPRL1. Binding of MA1-17769 was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (red). Cell nuclei are stained with DAPI (blue).
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||cDNA encoding human FPRL1|
|Storage buffer||PBS, pH 7.2|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1.2 µg/10^6 cells|
|Immunocytochemistry (ICC)||1 µg/10^6 cells|
|Immunofluorescence (IF)||1 µg/10^6 cells|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody recognizes native human FPRL1 protein expressed in transfected mammalian cells.
Human formyl peptide-receptor-like-1 (FPRL1) belongs to the large family of G-protein coupled receptors (GPCR). It is a seven transmembrane protein expressed on mononuclear phagocytes and microglial cells. FPRL1 is a member of the chemoattractant subfamily of G protein-coupled receptors and plays a key role in inflammation via chemotaxis and the regulation of mediator release from leukocytes. It interacts with formyl peptides to attract phagocytes to sites of infection and promote inflammatory reactions. FPRL1 also interacts with amyloid beta peptides and has been implicated in phagocyte attraction to sites of amyloid plaques in Alzheimer and quote;s disease. Since FPRL1 is expressed in neutrophils and monocytes, and it was shown using another monoclonal antibody that chemokines can be potent and specific ligands, FPRL1 might have interesting functions in inflammatory pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.