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FUS/TLS Monoclonal Antibody (3A10B5)

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FUS/TLS Antibody in Immunocytochemistry (ICC/IF) — IF result of McAB FUS (60160-1-Ig) in the Paper "FET proteins TAF15 and EWS are selective markers that distinguish FTLD with FUS pathology from amyotrophic lateral sclerosis with FUS mutations" from Manuela Neumann.

FUS/TLS Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 60160-1-IG ( FUS/TLS Antibody) at dilution of 1:1000 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

FUS/TLS Antibody in Immunohistochemistry (IHC) — IHC result of Monoclonal FUS antibody (60160-1-Ig) from Dr. Linda Kwong.

FUS/TLS Antibody in Western Blot (WB) — HL-60 cells were subjected to SDS PAGE followed by western blot with 60160-1-Ig (FUS/TLS Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

FUS/TLS Antibody in Flow Cytometry (Flow) — 1X10^6 K-562 cells were stained with 0.20ug FUS/TLS antibody (60160-1-IG, red) and control antibody (blue). Fixed with 90% MeOH.

FUS/TLS Antibody in Immunoprecipitation (IP) — IP result of anti-FUS/TLS (IP:60160-1-IG, 4ug; Detection:60160-1-IG 1:10000) with HeLa cells lysate 920ug.

Product Details

60160-1-IG

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 5 publications 5 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:500-1:2500	-
Immunocytochemistry (ICC/IF)	1:20-1:200	View 2 publications 2 publications
Flow Cytometry (Flow)	0.2 µg/1x10^6 cells	-
Immunoprecipitation (IP)	0.5-4.0 µg	-
Miscellaneous PubMed (Misc)	-	View 3 publications 3 publications
Immunohistochemistry (IHC)	-	View 2 publications 2 publications

Product Specifications
Species Reactivity	Human, Mouse, Pig, Rat
Published species	Fruit fly, Human
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	3A10B5
Immunogen	FUS/TLS Fusion Protein Ag2150 (52-400 aa encoded by BC026062) View immunogen
Conjugate	Unconjugated CoraLite488 CoraLite594
Form	Liquid
Concentration	1 mg/mL
Purification	Protein G
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

This antibody is a mouse monoclonal antibody raised against an internal region of human FUS. Initial reports from our customers suggest this new monoclonal FUS antibody (60160-1-Ig) is a useful tool in ALS and FTLD research. For more details, please see our blog article regarding the matter.

Immunogen sequence: QSSYSSYGQ SQNTGYGTQS TPQGYGSTGG YGSSQSSQSS YGQQSSYPGY GQQPAPSSTS GSYGSSSQSS SYGQPQSGSY SQQPSYGGQQ QSYGQQQSYN PPQGYGQQNQ YNSSSGGGGG GGGGGNYGQD QSSMSSGGGS GGGYGNQDQS GGGGSGGYGQ QDRGGRGRGG SGGGGGGGGG GYNRSSGGYE PRGRGGGRGG RGGMGGSDRG GFNKFGGPRD QGSRHDSEQD NSDNNTIFVQ GLGENVTIES VADYFKQIGI IKTNKKTGQP MINLYTDRET GKLKGEATVS FDDPPSAKAA IDWFDGKEFS GNPIKVSFAT RRADFNRGGG NGRGGRGRGG PMGRGGYGGG (52-400 aa encoded by BC026062)

Target Information

This gene encodes a multifunctional protein component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. The hnRNP complex is involved in pre-mRNA splicing and the export of fully processed mRNA to the cytoplasm. This protein belongs to the FET family of RNA-binding proteins which have been implicated in cellular processes that include regulation of gene expression, maintenance of genomic integrity and mRNA/microRNA processing. Alternative splicing results in multiple transcript variants. Defects in this gene result in amyotrophic lateral sclerosis type 6.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Self-assembly of FUS through its low-complexity domain contributes to neurodegeneration.

Human molecular genetics

Matsumoto T,Matsukawa K,Watanabe N,Kishino Y,Kunugi H,Ihara R,Wakabayashi T,Hashimoto T,Iwatsubo T

Sun Apr 15 00:00:00 EDT 2018

PINK1 and Parkin are genetic modifiers for FUS-induced neurodegeneration.

Human molecular genetics

Chen Y,Deng J,Wang P,Yang M,Chen X,Zhu L,Liu J,Lu B,Shen Y,Fushimi K,Xu Q,Wu JY

Thu Dec 01 00:00:00 EST 2016

A novel missense mutation of CMT2P alters transcription machinery.

Annals of neurology

Hu B,Arpag S,Zuchner S,Li J

Thu Dec 01 00:00:00 EST 2016

A novel missense mutation of CMT2P alters transcription machinery.

Annals of neurology

Hu B,Arpag S,Zuchner S,Li J

Thu Dec 01 00:00:00 EST 2016

Motor neuron degeneration in spastic paraplegia 11 mimics amyotrophic lateral sclerosis lesions.

Brain : a journal of neurology

Denora PS,Smets K,Zolfanelli F,Ceuterick-de Groote C,Casali C,Deconinck T,Sieben A,Gonzales M,Zuchner S,Darios F,Peeters D,Brice A,Malandrini A,De Jonghe P,Santorelli FM,Stevanin G,Martin JJ,El Hachimi KH

Wed Jun 01 00:00:00 EDT 2016

FUS Interacts with HSP60 to Promote Mitochondrial Damage.

PLoS genetics

Deng J,Yang M,Chen Y,Chen X,Liu J,Sun S,Cheng H,Li Y,Bigio EH,Mesulam M,Xu Q,Du S,Fushimi K,Zhu L,Wu JY

Tue Sep 01 00:00:00 EDT 2015

Hu antigen R (HuR) is a positive regulator of the RNA-binding proteins TDP-43 and FUS/TLS: implications for amyotrophic lateral sclerosis.

The Journal of biological chemistry

Lu L,Zheng L,Si Y,Luo W,Dujardin G,Kwan T,Potochick NR,Thompson SR,Schneider DA,King PH

Fri Nov 14 00:00:00 EST 2014

Poly-A binding protein-1 localization to a subset of TDP-43 inclusions in amyotrophic lateral sclerosis occurs more frequently in patients harboring an expansion in C9orf72.

Journal of neuropathology and experimental neurology

McGurk L,Lee VM,Trojanowksi JQ,Van Deerlin VM,Lee EB,Bonini NM

Mon Sep 01 00:00:00 EDT 2014

The RNA-binding protein Fus directs translation of localized mRNAs in APC-RNP granules.

The Journal of cell biology

Yasuda K,Zhang H,Loiselle D,Haystead T,Macara IG,Mili S

Mon Dec 09 00:00:00 EST 2013

The RNA-binding protein Fus directs translation of localized mRNAs in APC-RNP granules.

The Journal of cell biology

Yasuda K,Zhang H,Loiselle D,Haystead T,Macara IG,Mili S

Mon Dec 09 00:00:00 EST 2013

Topography of FUS pathology distinguishes late-onset BIBD from aFTLD-U.

Acta neuropathologica communications

Lee EB,Russ J,Jung H,Elman LB,Chahine LM,Kremens D,Miller BL,Branch Coslett H,Trojanowski JQ,Van Deerlin VM,McCluskey LF

Thu May 09 00:00:00 EDT 2013

Transportin 1 accumulates specifically with FET proteins but no other transportin cargos in FTLD-FUS and is absent in FUS inclusions in ALS with FUS mutations.

Acta neuropathologica

Neumann M,Valori CF,Ansorge O,Kretzschmar HA,Munoz DG,Kusaka H,Yokota O,Ishihara K,Ang LC,Bilbao JM,Mackenzie IR

Thu Nov 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: 16) in malignant liposarcoma); 16) malignant liposarcoma; 75 kDa DNA-pairing protein; fus-like protein; fused in sarcoma; fusion (involved in t(12; fusion gene in myxoid liposarcoma; fusion, derived from t(12; fusion, derived from t(12;16) malignant liposarcoma; heterogeneous nuclear ribonucleoprotein P2; hnRNP P2; Oncogene FUS; Oncogene TLS; pigpen protein; POMp75; Protein pigpen; RNA-binding protein FUS; translocated in liposarcoma; Translocated in liposarcoma protein

Gene Aliases: ALS6; D430004D17Rik; D930039C12Rik; ETM4; FUS; FUS1; HNRNPP2; POMP75; TLS

UniProt ID: (Human) P35637, (Mouse) P56959

Entrez Gene ID: (Pig) 100522185, (Human) 2521, (Rat) 317385, (Mouse) 233908

Molecular Function: RNA metabolism protein

Function(s)

mRNA splicing, via spliceosome cellular response to calcium ion regulation of nucleic acid-templated transcription biological_process

Process(es)

nucleotide binding DNA binding transcription coactivator activity RNA binding protein binding zinc ion binding estrogen receptor binding myosin V binding ionotropic glutamate receptor binding identical protein binding poly(A) RNA binding retinoid X receptor binding thyroid hormone receptor binding nucleic acid binding metal ion binding

FUS/TLS Monoclonal Antibody (3A10B5)

Product Details

60160-1-IG

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Immunohistochemistry (IHC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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