Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
Western blot analysis of FXR2 was performed by loading 20µg of the indicated whole cell lysates and 5µl of PageRuler Plus Prestained Protein Ladder (Product # 26619) onto a 4-20% Tris-Glycine polyacrylamide gel (Product # WT4202BX10). Proteins were transferred to a nitrocellulose membrane using the G2 Blotter (Product # 62288), and blocked with 5% Milk in TBST for 1 hour at room temperature. FXR2 was detected at ~95kDa using a FXR2 mouse monoclonal antibody (Product # MA1-16767) at a concentration of 1µg/ml in blocking buffer overnight at 4C on a rocking platform, followed by a Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177) at a dilution of 1:1000 for at least one hour at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34078). <br />
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant C-terminal fragment of human FXR2 (residues 414-658).|
|Contains||0.01% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||20 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||20 µg/ml|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Suggested positive control: Hela whole cell extract, HeLa whole cell lysate.
Fragile X syndrome is caused by the absence of the fragile X mental-retardation protein (FMRP). FMRP is the archetype of a class of cytoplasmic mRNA-binding proteins that includes the fragile X-related 1 and 2 proteins (FXR1 and FXR2). The fragile X-related proteins FXR1 and FXR2 contain a functional nucleolar-targeting signal equivalent to the HIV-1 regulatory proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
FMR1L2; fragile X mental retardation gene 2, autosomal homolog; Fragile X mental retardation syndrome-related protein 2; fragile X mental retardation, autosomal homolog 2; fragile X mental retardation-related protein 2; fragile X-mental retardation 1-like 2
FMR1L2; FXR2; Fxr2h; FXR2P