Immunofluorescent analysis of FXR2 in A431 cells. The cells were fixed with 100% methanol for 15 minutes at -20C, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 3% BSA for 30 minutes at room temperature. Cells were stained with a FXR2 mouse monoclonal antibody (Product # MA1-16767) at a concentration of 5ug/ml in blocking buffer overnight at 4C, and then incubated with a Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor® Plus 488 conjugate (Product # A32723) at a dilution of 1:500 for at least 30 minutes at a room temperature in the dark (green). Nuclei (blue) were stained with Hoechst 33342 (Product # 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant C-terminal fragment of human FXR2 (residues 414-658).|
|Contains||0.01% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||2.5-5 µg/ml|
|Immunofluorescence (IF)||2.5-5 µg/ml|
|Immunohistochemistry (IHC)||20 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||20 µg/ml|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 2 publications below|
Suggested positive control: Hela whole cell extract, HeLa whole cell lysate.
Fragile X syndrome is caused by the absence of the fragile X mental-retardation protein (FMRP). FMRP is the archetype of a class of cytoplasmic mRNA-binding proteins that includes the fragile X-related 1 and 2 proteins (FXR1 and FXR2). The fragile X-related proteins FXR1 and FXR2 contain a functional nucleolar-targeting signal equivalent to the HIV-1 regulatory proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Generation of monoclonal antibodies against phosphotyrosine and their use for affinity purification of phosphotyrosine-containing proteins.
MA1-16767 was used in ELISA and immunoprecipitation to investigate the specificity of phosphotyrosine antibodies in vitro
|Frackelton AR,Posner M,Kannan B,Mermelstein F||Methods in enzymology (201:79)||1991|
|Not Applicable||Not Cited||
Protein-tyrosine kinase activity and pp60v-src expression in whole cells measured by flow cytometry.
MA1-16767 was used in flow cytometry and immunohistochemistry to investigate the characteristics of pp60v-src in cells with flow cytometry
|Preis PN,Waldman FM,Frackelton AR,Saya H,Levin VA||Cancer research (48:4633)||1988|