|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Heavy chain of human factor IX and Ixa.|
|Purification||Ion-exchange chromatography, Ammonium sulfate precipitation|
|Storage buffer||50% water with 50% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||5-10 µg/mL|
|Immunohistochemistry (IHC)||Assay Dependent.|
|Radioimmune Assays (RIA)||Assay Dependent.|
|Western Blot (WB)||5 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-43012 detects Factor IX in human samples, human factor Ixa and the heavy chain of human factor IX and IXa.
MA1-43012 has successfully been used in radioimmune assays, ELISA, Western blot and immunohistochemistry applications.
MA1-43012 was derived from human factor IX.
Factor IX circulates in the blood as an inactive zymogen. This factor is converted to an active form by factor XIa, which excises the activation peptide and thus generates a heavy chain and a light chain held together by one or more disulfide bonds. The role of this activated factor IX in the blood coagulation cascade is to activate factor X to its active form through interactions with Ca+2 ions, membrane phospholipids, and factor VIII. Alterations of this gene, including point mutations, insertions and deletions, cause factor IX deficiency, which is a recessive X-linked disorder, also called hemophilia B or Christmas disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.