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|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A C-terminal region synthetic peptide made to the human FIH protein sequence (between residues 300 and the C-terminus).|
|Purification||Antigen affinity chromatography|
|Storage buffer||tris citrate, pH 7-8|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:50-1:200|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:200|
|Western Blot (WB)||1:1000-1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Suggested positive control: antigen standard for HIF1AN (transient overexpression lysate), rat astrocytes, C6 and U251 gliomas.
Factor Inhibiting HIF1 (FIH) protein facilitates repression of HIF-1 transcriptional activity by binding to VHL, which acts as a transcriptional corepressor. VHL inhibits HIF-1 alpha transactivation function by recruiting histone deacetylases. Involvement of VHL in association with FIH provides a unifying mechanism for the modulation of HIF-1 alpha protein stabilization and transcriptional activation in response to changes in cellular oxygen concentration.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
DKFZp762F1811; factor inhibiting HIF-1; factor inhibiting HIF1; FIH-1; FIH1; FLJ20615; FLJ22027; hypoxia inducible factor 1, alpha subunit inhibitor; hypoxia-inducible factor 1-alpha inhibitor; hypoxia-inducible factor asparagine hydroxylase; peptide-aspartate beta-dioxygenase
2310046M24Rik; A830014H24Rik; FIH; FIH1; HIF1AN