Keep as concentrated solution.
Positive Control: human F10-transfected 293T cells.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.
F10 undergoes multiple processing steps before its preproprotein is converted to a mature two-chain form by the excision of the tripeptide RKR. Two chains of the factor are held together by 1 or more disulfide bonds; the light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. The mature factor is activated by the cleavage of the activation peptide by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway). The activated factor then converts prothrombin to thrombin in the presence of factor Va, Ca+2, and phospholipid during blood clotting. Mutations of this gene result in factor X deficiency, a hemorrhagic condition of variable severity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Activated factor Xa heavy chain; Coagulation factor X; Factor X heavy chain; Factor X light chain; factor Xa; prothrombinase; Stuart factor; Stuart-Prower factor
Gene Aliases: F10; FX; FXA
UniProt ID: (Human) P00742
Entrez Gene ID: (Human) 2159
Molecular Function: annexin calcium-binding protein calmodulin enzyme modulator hydrolase intracellular calcium-sensing protein peptide hormone protease protease inhibitor receptor serine protease signaling molecule