Immunofluorescent analysis of Fatty Acid Synthase showing staining in the cytoplasm of 293T cells. 293T cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a Fatty Acid Synthase polyclonal antibody (Product # PA5-22111) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 401 and 694 of Fatty Acid Synthase (Uniprot ID#P49327)|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-22111 targets Fatty Acid Synthase in ICC, IF, IHC (P), and WB applications and shows reactivity with Human and mouse samples.
The PA5-22111 immunogen is a recombinant fragment corresponding to a region within amino acids 401 and 694 of Fatty Acid synthase (Uniprot ID#P49327).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.