Staining of normal human peripheral blood cells with Anti-Human IgE FITC (Product # 11-6986) and Mouse IgG2b K Isotype Control APC (Product # 17-4732) (left) or Anti-Human Fc epsilon Receptor I alpha (FceR1) APC (right). Cells in the lymphocyte gate were used for analysis (note: basophils reside in FCS/SSC position of lymphocytes).
|Tested species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG2b, kappa|
|Storage buffer||PBS, pH 7.2, with 0.1% gelatin, 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||5 µL (0.125 µg)/test|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Description: The AER-37 monoclonal antibody reacts with the Fc epsilon RI alpha subunit, an IgE-binding subunit lacking signal-transducing ability. Fc epsilon RI apha is expressed on mast and basophil cells and is upregulated by the presence of IgE. Fc epsilon RI alpha forms a tetrameric complex with one beta and two gamma subunits. The beta and gamma subunits possess immunoreceptor tyrosine-based activation motifs (ITAM). The Fc epsilon RI complex plays an important role in triggering IgE-mediated allergic reactions.
Applications Reported: The AER-37 (CRA1) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This AER-37 (CRA1) antibody has been pre-titrated and tested by flow cytometric analysis of peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser
The IgE receptor plays a central role in allergic disease, coupling allergen and mast cell to initiate the inflammatory and immediate hypersensitivity responses that are characteristic of disorders such as hay fever and asthma. The allergic response occurs when 2 or more high-affinity IgE receptors are crosslinked via IgE molecules that in turn are bound to an allergen (antigen) molecule. A perturbation occurs that brings about the release of histamine and proteases from the granules in the cytoplasm of the mast cell and leads to the synthesis of prostaglandins and leukotrienes--potent effectors of the hypersensitivity response. The IgE receptor consists of 3 subunits: alpha, beta ., and gamma .; only the alpha subunit is glycosylated.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||IgE- and FcepsilonRI-mediated migration of human basophils.||Suzukawa M,Hirai K,Iikura M,Nagase H,Komiya A,Yoshimura-Uchiyama C,Yamada H,Ra C,Ohta K,Yamamoto K,Yamaguchi M||International immunology (17:1249)||2005|
|Not Applicable||Not Cited||Regulation of the human Fc epsilon RI alpha-chain distal promoter.||Hasegawa M,Nishiyama C,Nishiyama M,Akizawa Y,Takahashi K,Ito T,Furukawa S,Ra C,Okumura K,Ogawa H||Journal of immunology (Baltimore, Md. : 1950) (170:3732)||2003|
|Not Applicable||Not Cited||Functional expression of the high affinity receptor for IgE (FcepsilonRI) in human platelets and its' intracellular expression in human megakaryocytes.||Hasegawa S,Pawankar R,Suzuki K,Nakahata T,Furukawa S,Okumura K,Ra C||Blood (93:2543)||1999|
|Not Applicable||Not Cited||Soluble human high-affinity receptor for IgE abrogates the IgE-mediated allergic reaction.||Ra C,Kuromitsu S,Hirose T,Yasuda S,Furuichi K,Okumura K||International immunology (5:47)||1993|
|Not Applicable||Not Cited||The alpha subunit of the human IgE receptor (FcERI) is sufficient for high affinity IgE binding.||Hakimi J,Seals C,Kondas JA,Pettine L,Danho W,Kochan J||The Journal of biological chemistry (265:22079)||1990|
|Not Applicable||Not Cited||IgE enhances Fc epsilon receptor I expression and IgE-dependent release of histamine and lipid mediators from human umbilical cord blood-derived mast cells: synergistic effect of IL-4 and IgE on human mast cell Fc epsilon receptor I expression and mediator release.||Yamaguchi M,Sayama K,Yano K,Lantz CS,Noben-Trauth N,Ra C,Costa JJ,Galli SJ||Journal of immunology (Baltimore, Md. : 1950) (162:5455)||1999|