Invitrogen

FceR1 alpha Monoclonal Antibody (AER-37 (CRA1)), APC, eBioscience™

Product Image Gallery

FceR1 alpha Antibody in Flow Cytometry (Flow) — Staining of normal human peripheral blood cells with Anti-Human IgE FITC (Product # 11-6986-42) and Mouse IgG2b K Isotype Control APC (Product # 17-4732-81) (left) or Anti-Human Fc epsilon Receptor I alpha (FceR1) APC (right). Cells in the lymphocyte gate were used for analysis (note: basophils reside in FCS/SSC position of lymphocytes).

Product Details

17-5899-42

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	5 µL (0.125 µg)/test	View 10 publications 10 publications

Product Specifications
Species Reactivity	Human
Published species	Human, Mouse
Host/Isotype	Mouse / IgG2b, kappa
Recommended Isotype Control	Mouse IgG2b kappa Isotype Control (eBMG2b), APC, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	AER-37 (CRA1)
Conjugate	APC Biotin eFluor 450 FITC Functional Grade NovaFluor Blue 610-70S NovaFluor Blue 660-120S NovaFluor Yellow 570 NovaFluor Yellow 610 NovaFluor Yellow 660 PE PE-Cyanine7 PE-eFluor 610 PerCP-eFluor 710 Super Bright 436 Super Bright 600 Super Bright 780 Unconjugated
Excitation/Emission Profile	View spectra
Form	Liquid
Concentration	5 µL/Test
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2, with 0.1% gelatin, 0.2% BSA
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_10671394

Product Specific Information

Description: The AER-37 monoclonal antibody reacts with the Fc epsilon RI alpha subunit, an IgE-binding subunit lacking signal-transducing ability. Fc epsilon RI apha is expressed on mast and basophil cells and is upregulated by the presence of IgE. Fc epsilon RI alpha forms a tetrameric complex with one beta and two gamma subunits. The beta and gamma subunits possess immunoreceptor tyrosine-based activation motifs (ITAM). The Fc epsilon RI complex plays an important role in triggering IgE-mediated allergic reactions.

Applications Reported: The AER-37 (CRA1) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This AER-37 (CRA1) antibody has been pre-titrated and tested by flow cytometric analysis of peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

FceR1 (Fc epsilonR1 alpha) is a subunit of the high affinity receptor of IgE. Fc epsilonR1 alpha is a tetrameric complex consisting of one alpha, one ß and two additional subunits. The latter two are required for signal transduction activity. The Fc epsilonR1 complex plays an important role in triggering allergic responses, and the immunoglobulin epsilon receptor (IgE receptor) is the initiator of the allergic response. When two or more high-affinity IgE receptors are brought together by allergen-bound IgE molecules, mediators such as histamine, which are responsible for allergy symptoms, are released. The IgE receptor couples allergen and mast cells to initiate the inflammatory responses that are characteristic of disorders such as hay fever and asthma. The release of histamine and proteases leads to the synthesis of prostaglandins and leukotrienes-potent effectors of the hypersensitivity response.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

The mechanistic and functional profile of the therapeutic anti-IgE antibody ligelizumab differs from omalizumab.

Nature communications

Gasser P,Tarchevskaya SS,Guntern P,Brigger D,Ruppli R,Zbären N,Kleinboelting S,Heusser C,Jardetzky TS,Eggel A

17-5899 was used in Flow cytometry/Cell sorting to investigate and compare the molecular binding profile and functional mechanisms of action of the anti-IgE antibody ligelizumab and omalizumab.

Wed Jan 08 00:00:00 EST 2020

Identification of TNFR2 and IL-33 as therapeutic targets in localized fibrosis.

Science advances

Izadi D,Layton TB,Williams L,McCann F,Cabrita M,Espirito Santo AI,Xie W,Fritzsche M,Colin-York H,Feldmann M,Midwood KS,Nanchahal J

Published figure using FceR1 alpha monoclonal antibody (Product # 17-5899-42) in Flow Cytometry

Sun Dec 01 00:00:00 EST 2019

Structural basis of omalizumab therapy and omalizumab-mediated IgE exchange.

Nature communications

Pennington LF,Tarchevskaya S,Brigger D,Sathiyamoorthy K,Graham MT,Nadeau KC,Eggel A,Jardetzky TS

17-5899 was used in Flow cytometry/Cell sorting to discover a treatment blocking basophil activation and provide a novel approach to probe mechanisms underlying IgE hypersensitivity.

Thu May 19 00:00:00 EDT 2016

A pilot study of omalizumab in eosinophilic esophagitis.

PloS one

Loizou D,Enav B,Komlodi-Pasztor E,Hider P,Kim-Chang J,Noonan L,Taber T,Kaushal S,Limgala R,Brown M,Gupta R,Balba N,Goker-Alpan O,Khojah A,Alpan O

17-5899 was used in Flow cytometry/Cell sorting to demonstrate that anti-IgE therapy with omalizumab may result in Eosinophilic Esophagitis disease remission.

Thu Feb 04 00:00:00 EST 2016

Human Mucosal Mast Cells Capture HIV-1 and Mediate Viral trans-Infection of CD4+ T Cells.

Journal of virology

Jiang AP,Jiang JF,Wei JF,Guo MG,Qin Y,Guo QQ,Ma L,Liu BC,Wang X,Veazey RS,Ding YB,Wang JH

17-5899 was used in Flow cytometry/Cell sorting to investigate the role of mast cells in HIV-1 infection.

Wed Dec 30 00:00:00 EST 2015

Human Blood-Circulating Basophils Capture HIV-1 and Mediate Viral trans-Infection of CD4+ T Cells.

Journal of virology

Jiang AP,Jiang JF,Guo MG,Jin YM,Li YY,Wang JH

17-5899 was used in Flow cytometry/Cell sorting to investigate the role of human granulocytes in the dissemination of HIV-1.

Sat Aug 01 00:00:00 EDT 2015

Dendritic cell-bound IgE functions to restrain allergic inflammation at mucosal sites.

Mucosal immunology

Platzer B,Baker K,Vera MP,Singer K,Panduro M,Lexmond WS,Turner D,Vargas SO,Kinet JP,Maurer D,Baron RM,Blumberg RS,Fiebiger E

17-5899 was used in Flow cytometry/Cell sorting to confirm a novel immune regulatory function of Immunoglobulin E.

Fri May 01 00:00:00 EDT 2015

MHC class II expression in human basophils: induction and lack of functional significance.

PloS one

Voskamp AL,Prickett SR,Mackay F,Rolland JM,O'Hehir RE

17-5899 was used in Flow cytometry/Cell sorting to determine the antigen-presenting abilities of basophils and their role in initiating a Th2 phenotype.

Mon Sep 29 00:00:00 EDT 2014

Generation, isolation, and maintenance of human mast cells and mast cell lines derived from peripheral blood or cord blood.

Current protocols in immunology

Rådinger M,Jensen BM,Kuehn HS,Kirshenbaum A,Gilfillan AM

17-5899 was used in Flow cytometry/Cell sorting to describe the relative merits and drawbacks of techniques for the development and culture of human mast cells.

Sun Aug 01 00:00:00 EDT 2010

The signal peptide of the IgE receptor alpha-chain prevents surface expression of an immunoreceptor tyrosine-based activation motif-free receptor pool.

The Journal of biological chemistry

Platzer B,Fiebiger E

17-5899 was used in Flow cytometry/Cell sorting to investigate the role of FcepsilonRI alpha-chain in immune response towards allergens.

Fri May 14 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: fc epsilon r1; Fc epsilon receptor Ia; Fc epsilon RI alpha-chain; Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide; Fc IgE receptor, alpha polypeptide; Fc-epsilon RI-alpha; FcERI; high affinity immunoglobulin epsilon receptor alpha-subunit; High affinity immunoglobulin epsilon receptor subunit alpha; IgE Fc receptor subunit alpha; immunoglobulin E receptor, high-affinity, of mast cells, alpha polypeptide; RP11-550P17.3

Gene Aliases: FCE1A; FCER1A; FcERI

UniProt ID: (Human) P12319

Entrez Gene ID: (Human) 2205

Molecular Function: immunoglobulin receptor superfamily