Invitrogen

FceR1 alpha Monoclonal Antibody (MAR-1), Functional Grade, eBioscience™

FceR1 alpha Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 23

FceR1 alpha Antibody (16-5898-82) in Flow

Staining of MC/9 cell line with 0.125 µg of Armenian Hamster IgG Isotype Control Functional Grade Purified (Product # 16-4888-81) (open histogram) or 0.125 µg of Anti-Mouse Fc epsilon Receptor I alpha (Fc epsilon R1) Functional Grade Purified (filled histogram) followed by Anti-Armenian Hamster IgG FITC (Product # 11-4111).Total viable cells were used for analysis.

Product Image Gallery

FceR1 alpha Antibody in Immunocytochemistry (ICC/IF) — Published figure using FceR1 alpha monoclonal antibody (Product # 16-5898-82) in Immunocytochemistry

FceR1 alpha Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow) — Published figure using FceR1 alpha monoclonal antibody (Product # 16-5898-82) in Immunocytochemistry and Flow Cytometry

FceR1 alpha Antibody in Immunohistochemistry (IHC) — Published figure using FceR1 alpha monoclonal antibody (Product # 16-5898-82) in Immunohistochemistry

FceR1 alpha Antibody in Western Blot (WB) — Fig. 4 Role of macrophage Nhe1 in atherosclerosis. Quantification of aortic root pHrodo-positive area, intima area, Mac-3 + macrophage content, TUNEL-positive apoptotic cell number, and alpha-actin-positive SMC content in Apoe -/- mice received bone-marrow from Apoe -/- Nhe1 +/+ ( n = 7) and Apoe -/- Nhe1 +/- ( n = 10) donor mice a or received bone-marrow from Apoe -/- Fcer1a +/+ ( n = 8) and Apoe -/- Fcer1a -/- ( n = 11) donor mice b as indicated. Representative images of macrophage staining and pHrodo detection for panels a and b are shown to the left. Bars: 500 um, inset bars: 200 um. Data are mean +- SEM. Mann-Whitney U- test (intima area in b ) and two-tailed Student's t test (all other panels) were used for statistic analyses. * P < 0.05; ** P < 0.01; *** P < 0.001. Source data are provided as a Source Data file. c Immunoprecipitation (IP) and immunoblot (IB) analysis of macrophages from WT and Fcer1a -/- mice after cells are treated with or without IgE. Cell lysate IP is performed using anti-Nhe1 antibody and IB is performed using either anti-FcepsilonR1 antibody (top panel) or directly with HRP-conjugated anti-IgG antibody (bottom panel). Input cell lysate from IgE-treated WT macrophages is used as positive control. d Immunofluorescent triple staining for Mac-2 macrophages, FcepsilonR1, and Nhe1 in atherosclerotic lesions from Apoe -/- Nhe1 +/+ mice ( n = 9). White arrows indicate FcepsilonR1 and Nhe1 colocalization on Mac-2 + macrophages. Parallel sections of panel d

FceR1 alpha Antibody in Western Blot, Immunoprecipitation (WB, IP) — Figure 2 Negative and positive regulatory roles of C-terminal Src kinase (CSK) in tyrosine phosphorylation of early signal-transduction molecules. (A) Total tyrosine phosphorylation of IgE-sensitized bone marrow-derived mast cells (BMMCs) with CSK-KD or control pLKO.1 cells activated or not with antigen (250 ng/ml) for various time intervals. The cells were lysed and analyzed by immunoblotting (IB) with tyrosine-specific monoclonal antibodies PY20-HRP conjugate (PY). GRB2 was used as a loading control. Representative immunoblots from three experiments are shown. Numbers on the right indicate positions of molecular weight standards. (B) Densitometry analysis of the immunoblots as from panel (A) . (C) FceRIs were immunoprecipitated (IP) from the lysates of nonactivated or antigen-activated BMMCs with CSK-KD or control cells (pLKO.1). The immunoprecipitates were examined by immunoblotting with PY20-HRP conjugate. For loading controls, FceRI beta chain-specific antibody was used. Positions of FceRI beta and gamma chains are marked by arrows. A representative immunoblot from three performed is shown. (D) Densitometry analysis of the immunoblots as from panel (C) , in which pooled signals from tyrosine-phosphorylated FcepsilonRI beta and gamma chains in activated cells were normalized to the signals from nonactivated cells and loading control protein (FcepsilonRI beta chain). Means +- SEM were calculated from three independent experiments. (E-M) IgE-sensitized BMMCs with CSK-KD or

Product Details

16-5898-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.25 µg/test

View 13 publications 13 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

Neutralization (Neu)

Assay-Dependent

Functional Assay (FN)

Assay-Dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MAR-1

Conjugate

Functional Grade

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

4° C

RRID

AB_469137

Product Specific Information

Description: The MAR-1 monoclonal antibody reacts with the Fc epsilon Receptor I alpha subunit, an IgE-binding subunit lacking signal-transducing ability. Fc epsilon RI alpha; is expressed on mast and basophil cells and is up-regulated by the presence of IgE. Fc epsilon RI alpha forms a tetrameric complex with one beta and two gamma subunits. The beta and gamma subunits possess immunoreceptor tyrosine-based activation motifs (ITAM). The Fc epsilon RI complex plays an important role in triggering IgE-mediated allergic reactions.

Applications Reported: The MAR-1 antibody has been reported for use in flow cytometric analysis. It has also been reported in blocking of IgE to Fc epsilon Receptor and degranulation of mast cells.

Applications Tested: The MAR-1 antibody has been tested by flow cytometric analysis of the MC/9 cell line (a mouse mast cell line). This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

FceR1 (Fc epsilonR1 alpha) is a subunit of the high affinity receptor of IgE. Fc epsilonR1 alpha is a tetrameric complex consisting of one alpha, one ß and two additional subunits. The latter two are required for signal transduction activity. The Fc epsilonR1 complex plays an important role in triggering allergic responses, and the immunoglobulin epsilon receptor (IgE receptor) is the initiator of the allergic response. When two or more high-affinity IgE receptors are brought together by allergen-bound IgE molecules, mediators such as histamine, which are responsible for allergy symptoms, are released. The IgE receptor couples allergen and mast cells to initiate the inflammatory responses that are characteristic of disorders such as hay fever and asthma. The release of histamine and proteases leads to the synthesis of prostaglandins and leukotrienes-potent effectors of the hypersensitivity response.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

NLRP3 Inflammasome Activation of Mast Cells by Estrogen via the Nuclear-Initiated Signaling Pathway Contributes to the Development of Endometriosis.

Frontiers in immunology

Guo X,Xu X,Li T,Yu Q,Wang J,Chen Y,Ding S,Zhu L,Zou G,Zhang X

Published figure using FceR1 alpha monoclonal antibody (Product # 16-5898-82) in Flow Cytometry

Tue Jan 18 00:00:00 EST 2022

SNAP23 is essential for platelet and mast cell development and required in connective tissue mast cells for anaphylaxis.

The Journal of biological chemistry

Cardenas RA,Gonzalez R,Sanchez E,Ramos MA,Cardenas EI,Rodarte AI,Alcazar-Felix RJ,Isaza A,Burns AR,Heidelberger R,Adachi R

Published figure using FceR1 alpha monoclonal antibody (Product # 16-5898-82) in Flow Cytometry

Mon Aug 02 00:00:00 EDT 2021

Mutant Huntingtin affects toll-like receptor 4 intracellular trafficking and cytokine production in mast cells.

Journal of neuroinflammation

Pérez-Rodríguez MJ,Ibarra-Sánchez A,Román-Figueroa A,Pérez-Severiano F,González-Espinosa C

Published figure using FceR1 alpha monoclonal antibody (Product # 16-5898-82) in Flow Cytometry

Fri Mar 27 00:00:00 EDT 2020

Tespa1 plays a role in the modulation of airway hyperreactivity through the IL-4/STAT6 pathway.

Journal of translational medicine

Yang R,Wang G,Li L,He H,Zheng M,Lu L,Wu S

Published figure using FceR1 alpha monoclonal antibody (Product # 16-5898-82) in Flow Cytometry

Mon Nov 23 00:00:00 EST 2020

Type I interferon limits mast cell-mediated anaphylaxis by controlling secretory granule homeostasis.

PLoS biology

Kobayashi T,Shimabukuro-Demoto S,Tsutsui H,Toyama-Sorimachi N

Published figure using FceR1 alpha monoclonal antibody (Product # 16-5898-82) in Flow Cytometry

Fri Nov 01 00:00:00 EDT 2019

Na⁺-H⁺ exchanger 1 determines atherosclerotic lesion acidification and promotes atherogenesis.

Nature communications

Liu CL,Zhang X,Liu J,Wang Y,Sukhova GK,Wojtkiewicz GR,Liu T,Tang R,Achilefu S,Nahrendorf M,Libby P,Guo J,Zhang JY,Shi GP

Published figure using FceR1 alpha monoclonal antibody (Product # 16-5898-82) in Western Blot

Wed Sep 04 00:00:00 EDT 2019

Positive and Negative Regulatory Roles of C-Terminal Src Kinase (CSK) in FcεRI-Mediated Mast Cell Activation, Independent of the Transmembrane Adaptor PAG/CSK-Binding Protein.

Frontiers in immunology

Potuckova L,Draberova L,Halova I,Paulenda T,Draber P

Published figure using FceR1 alpha monoclonal antibody (Product # 16-5898-82) in Flow Cytometry

Mon Oct 07 00:00:00 EDT 2019

Positive and Negative Regulatory Roles of C-Terminal Src Kinase (CSK) in FcεRI-Mediated Mast Cell Activation, Independent of the Transmembrane Adaptor PAG/CSK-Binding Protein.

Frontiers in immunology

Potuckova L,Draberova L,Halova I,Paulenda T,Draber P

Published figure using FceR1 alpha monoclonal antibody (Product # 16-5898-82) in Flow Cytometry

Mon Oct 07 00:00:00 EDT 2019

Positive and Negative Regulatory Roles of C-Terminal Src Kinase (CSK) in FcεRI-Mediated Mast Cell Activation, Independent of the Transmembrane Adaptor PAG/CSK-Binding Protein.

Frontiers in immunology

Potuckova L,Draberova L,Halova I,Paulenda T,Draber P

Published figure using FceR1 alpha monoclonal antibody (Product # 16-5898-82) in Flow Cytometry

Mon Oct 07 00:00:00 EDT 2019

Mast cell-derived neurotrophin 4 mediates allergen-induced airway hyperinnervation in early life.

Mucosal immunology

Patel KR,Aven L,Shao F,Krishnamoorthy N,Duvall MG,Levy BD,Ai X

Published figure using FceR1 alpha monoclonal antibody (Product # 16-5898-82) in Flow Cytometry

Tue Nov 01 00:00:00 EDT 2016

New Regulatory Roles of Galectin-3 in High-Affinity IgE Receptor Signaling.

Molecular and cellular biology

Bambouskova M,Polakovicova I,Halova I,Goel G,Draberova L,Bugajev V,Doan A,Utekal P,Gardet A,Xavier RJ,Draber P

Published figure using FceR1 alpha monoclonal antibody (Product # 16-5898-82) in Immunofluorescence

Sun May 01 00:00:00 EDT 2016

New Regulatory Roles of Galectin-3 in High-Affinity IgE Receptor Signaling.

Molecular and cellular biology

Bambouskova M,Polakovicova I,Halova I,Goel G,Draberova L,Bugajev V,Doan A,Utekal P,Gardet A,Xavier RJ,Draber P

Published figure using FceR1 alpha monoclonal antibody (Product # 16-5898-82) in Immunofluorescence

Sun May 01 00:00:00 EDT 2016

Isolation of Mature (Peritoneum-Derived) Mast Cells and Immature (Bone Marrow-Derived) Mast Cell Precursors from Mice.

PloS one

Meurer SK,Neß M,Weiskirchen S,Kim P,Tag CG,Kauffmann M,Huber M,Weiskirchen R

Published figure using FceR1 alpha monoclonal antibody (Product # 16-5898-82) in Flow Cytometry

Mon Jul 24 00:00:00 EDT 2017

Deficiency of FcϵR1 Increases Body Weight Gain but Improves Glucose Tolerance in Diet-Induced Obese Mice.

Endocrinology

Lee YJ,Liu C,Liao M,Sukhova GK,Shirakawa J,Abdennour M,Iamarene K,Andre S,Inouye K,Clement K,Kulkarni RN,Banks AS,Libby P,Shi GP

Published figure using FceR1 alpha monoclonal antibody (Product # 16-5898-82) in Western Blot

Sun Nov 01 00:00:00 EDT 2015

A next-generation dual-recombinase system for time- and host-specific targeting of pancreatic cancer.

Nature medicine

Schönhuber N,Seidler B,Schuck K,Veltkamp C,Schachtler C,Zukowska M,Eser S,Feyerabend TB,Paul MC,Eser P,Klein S,Lowy AM,Banerjee R,Yang F,Lee CL,Moding EJ,Kirsch DG,Scheideler A,Alessi DR,Varela I,Bradley A,Kind A,Schnieke AE,Rodewald HR,Rad R,Schmid RM,Schneider G,Saur D

Published figure using FceR1 alpha monoclonal antibody (Product # 16-5898-82) in Immunofluorescence

Sat Nov 01 00:00:00 EDT 2014

MHC class II-dependent basophil-CD4+ T cell interactions promote T(H)2 cytokine-dependent immunity.

Nature immunology

Perrigoue JG,Saenz SA,Siracusa MC,Allenspach EJ,Taylor BC,Giacomin PR,Nair MG,Du Y,Zaph C,van Rooijen N,Comeau MR,Pearce EJ,Laufer TM,Artis D

Published figure using FceR1 alpha monoclonal antibody (Product # 16-5898-82) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2009

MHC class II-dependent basophil-CD4+ T cell interactions promote T(H)2 cytokine-dependent immunity.

Nature immunology

Perrigoue JG,Saenz SA,Siracusa MC,Allenspach EJ,Taylor BC,Giacomin PR,Nair MG,Du Y,Zaph C,van Rooijen N,Comeau MR,Pearce EJ,Laufer TM,Artis D

Published figure using FceR1 alpha monoclonal antibody (Product # 16-5898-82) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2009

IL-21R is essential for epicutaneous sensitization and allergic skin inflammation in humans and mice.

The Journal of clinical investigation

Jin H,Oyoshi MK,Le Y,Bianchi T,Koduru S,Mathias CB,Kumar L,Le Bras S,Young D,Collins M,Grusby MJ,Wenzel J,Bieber T,Boes M,Silberstein LE,Oettgen HC,Geha RS

Published figure using FceR1 alpha monoclonal antibody (Product # 16-5898-82) in Flow Cytometry

Thu Jan 01 00:00:00 EST 2009

Mast cell degranulation breaks peripheral tolerance.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

de Vries VC,Wasiuk A,Bennett KA,Benson MJ,Elgueta R,Waldschmidt TJ,Noelle RJ

16-5898 was used in Flow cytometry/Cell sorting to show that intragraft or systemic mast cell degranulation results in the transient loss of Treg suppressor activities and the rejection of established allografts.

Thu Oct 01 00:00:00 EDT 2009

Developmental checkpoints of the basophil/mast cell lineages in adult murine hematopoiesis.

Proceedings of the National Academy of Sciences of the United States of America

Arinobu Y,Iwasaki H,Gurish MF,Mizuno S,Shigematsu H,Ozawa H,Tenen DG,Austen KF,Akashi K

Published figure using FceR1 alpha monoclonal antibody (Product # 16-5898-82) in Flow Cytometry

Tue Dec 13 00:00:00 EST 2005

Bioinformatics

Protein Aliases: fc epsilon r1; Fc-epsilon RI-alpha; FcERI; High affinity immunoglobulin epsilon receptor subunit alpha; IgE Fc receptor subunit alpha; RP11-550P17.3

Gene Aliases: Fce1a; fcepsilonri; Fcer1a; FcERI; Fcr-5

UniProt ID: (Mouse) P20489

Entrez Gene ID: (Mouse) 14125

Molecular Function: immunoglobulin receptor superfamily

Function(s)

activation of MAPK activity positive regulation of type I hypersensitivity serotonin secretion signal transduction cell surface receptor signaling pathway activation of JUN kinase activity leukotriene biosynthetic process Fc-epsilon receptor signaling pathway positive regulation of mast cell degranulation positive regulation of interleukin-3 biosynthetic process positive regulation of granulocyte macrophage colony-stimulating factor biosynthetic process positive regulation of peptidyl-tyrosine phosphorylation positive regulation of calcium-mediated signaling

Process(es)

protein binding IgE receptor activity IgE binding

FceR1 alpha Monoclonal Antibody (MAR-1), Functional Grade, eBioscience™

FceR1 alpha Antibody (16-5898-82) in Flow

Product Details

16-5898-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Neutralization (Neu)

Functional Assay (FN)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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