|Tested species reactivity||Virus|
|Host / Isotype||Mouse / IgG1|
|Storage buffer||PBS, pH 7.2|
|Contains||0.05% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||1:1,500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
In Western blot, this antibody detects a band at ~28kDa under reducing conditions. A strong band of 80kDa representing glycosylated FeLV gag gene product may also be present.
Feline leukemia virus (FeLV) is a retrovirus that infects cats. As a retrovirus, FeLV is transmitted as an RNA virus but the RNA is reverse transcribed into DNA. Retroviruses contradict the Central dogma of molecular biology. FeLV is usually transmitted between infected cats when the transfer of saliva or nasal secretions is involved. If not defeated by the animalâ€™s immune system, the virus can be lethal. The disease caused by this virus is a form of cancer of blood cells called lymphocytes (a leukemia).
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