|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human Fetal Hemoglobin (HbF)|
|Storage buffer||PBS with BSA, <10% sucrose|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 3 publications below|
Commonly used, FITC conjugates provide relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility.
This Fetal Hemoglobin monoclonal antibody specifically recognizes fetal hemoglobin found in fetal erythrocytes.
The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported.
Analyte Specific Reagent
Chemical Inhibition of Histone Deacetylases 1 and 2 Induces Fetal Hemoglobin through Activation of GATA2.
MHFH01 was used in flow cytometry to demonstrate that chemical inhibition of HDAC1/2 induces gamma-globin and propose a role for the GATA2 gene in this process
|Shearstone JR,Golonzhka O,Chonkar A,Tamang D,van Duzer JH,Jones SS,Jarpe MB||PloS one (11:null)||2016|
Extensive ex vivo expansion of functional human erythroid precursors established from umbilical cord blood cells by defined factors.
MHFH01 was used in flow cytometry to describe a method to generate red blood cells ex vivo using human erythroid precursors
|Huang X,Shah S,Wang J,Ye Z,Dowey SN,Tsang KM,Mendelsohn LG,Kato GJ,Kickler TS,Cheng L||Molecular therapy : the journal of the American Society of Gene Therapy (22:451)||2014|
Development of phenotypic screening assays for ¿-globin induction using primary human bone marrow day 7 erythroid progenitor cells.
MHFH01 was used in flow cytometry to develop a method using primary human bone marrow-derived erythroid progenitor cells to screen for compounds that induce γ-globin production.
|Li H,Xie W,Gore ER,Montoute MN,Bee WT,Zappacosta F,Zeng X,Wu Z,Kallal L,Ames RS,Pope AJ,Benowitz A,Erickson-Miller CL||Journal of biomolecular screening (18:1212)||2013|