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Immunofluorescent analysis of Fibrillin-1 (green) showing staining in the cytoplasm of SH-SY5Y cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Fibrillin-1 monoclonal antibody (Product # MA5-12770) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Bovine, Human|
|Published species reactivity||Pig, Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Microfibrils from zonular apparatus of bovine eye|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunomicroscopy (IM)||Assay Dependent|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-12770 targets Fibrillin-1 in ICC/IF, IHC (F), IM, and WB applications and shows reactivity with Bovine and Human samples.
The MA5-12770 immunogen is microfibrils from zonular apparatus of bovine eye.
Fibrillin-1 is an extracellular acidic protein with a high cysteine content and an extended thread-like shape with mosaic composition of different types of extracellular modules. Most of the fibrillin molecule is contributed by 47 epidermal growth factor-like (EGF-like) repeats; 43 of them have a consensus sequence for calcium binding (cb). Other modules including 8-cysteine motifs (seven), hybrid motifs (two), and unique domains (three) are interspersed throughout the molecule[3-7]. fibrillin-1 contains a proline-rich region close to the N-terminus that is replaced by a glycine-rich region in fibrillin-2. The fibrillins are found throughout the connective tissue as integral components of extended fibrils.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Geographical ancestry is a key determinant of epidermal morphology and dermal composition.
MA5-12770 was used in immunohistochemistry to study epidermal morphology and dermal composition in individuals of diverese geographic ancestry
|Langton AK,Sherratt MJ,Sellers WI,Griffiths CE,Watson RE||The British journal of dermatology (171:274)||2014|
Latent transforming growth factor-β binding protein 2 negatively regulates coalescence of oxytalan fibers induced by stretching stress.
MA5-12770 was used in immunohistochemistry to study the mechanism by which LTBP-2 modulates the coalescence of oxytalan fibres
|Tsuruga E,Oka K,Hatakeyama Y,Isokawa K,Sawa Y||Connective tissue research (53:521)||2012|
Tomato paste rich in lycopene protects against cutaneous photodamage in humans in vivo: a randomized controlled trial.
MA5-12770 was used in immunohistochemistry to study the ability of tomato paste rich in lycopene to protect against cutaneous photodamage in humans
|Rizwan M,Rodriguez-Blanco I,Harbottle A,Birch-Machin MA,Watson RE,Rhodes LE||The British journal of dermatology (164:154)||2011|
Effects of a cosmetic 'anti-ageing' product improves photoaged skin [corrected].
MA5-12770 was used in immunohistochemistry to study the effects of a cosmetic 'anti-ageing' product on fibrillin-1 deposition and repair of photoaged skin
|Watson RE,Ogden S,Cotterell LF,Bowden JJ,Bastrilles JY,Long SP,Griffiths CE||The British journal of dermatology (161:419)||2009|
Increased expression of extracellular matrix proteins in rapid atrial pacing-induced atrial fibrillation.
MA5-12770 was used in immunohistochemistry to examine the change of extracellular matrix proteins during atrial fibrillation
|Lin CS,Lai LP,Lin JL,Sun YL,Hsu CW,Chen CL,Mao SJ,Huang SK||Heart rhythm : the official journal of the Heart Rhythm Society (4:938)||2007|
Photoprotective and anti-skin-aging effects of eicosapentaenoic acid in human skin in vivo.
MA5-12770 was used in immunohistochemistry to investigate the protective effect of eicosapentaenoic acid against UV-induced skin damage and intrinsic aging
|Kim HH,Cho S,Lee S,Kim KH,Cho KH,Eun HC,Chung JH||Journal of lipid research (47:921)||2006|
The effects of a novel synthetic retinoid, seletinoid G, on the expression of extracellular matrix proteins in aged human skin in vivo.
MA5-12770 was used in immunohistochemistry to study the therapeutic benefits of topical application of the synthetic seletinoid G
|Kim MS,Lee S,Rho HS,Kim DH,Chang IS,Chung JH||Clinica chimica acta; international journal of clinical chemistry (362:161)||2005|
Sex steroids modulate human aortic smooth muscle cell matrix protein deposition and matrix metalloproteinase expression.
MA5-12770 was used in immunohistochemistry to study the role of sex steroids in modulating matrix protein deposition and MMP expression in human aortic smooth muscle cells
|Natoli AK,Medley TL,Ahimastos AA,Drew BG,Thearle DJ,Dilley RJ,Kingwell BA||Hypertension (Dallas, Tex. : 1979) (46:1129)||2005|
Ramipril reduces large-artery stiffness in peripheral arterial disease and promotes elastogenic remodeling in cell culture.
MA5-12770 was used in immunohistochemistry to study the mechanisms by which ramipril improves cardiovascular outcome in patients with peripheral arterial disease
|Ahimastos AA,Natoli AK,Lawler A,Blombery PA,Kingwell BA||Hypertension (Dallas, Tex. : 1979) (45:1194)||2005|
Abnormal hepatic expression of fibrillin-1 in children with cholestasis.
MA5-12770 was used in immunohistochemistry to study the expression of fibrillin-1 in children with cholestasis
|Lamireau T,Dubuisson L,Lepreux S,Bioulac-Sage P,Fabre M,Rosenbaum J,Desmoulière A||The American journal of surgical pathology (26:637)||2002|
Microfibril-associated glycoprotein-1 controls human ciliary zonule development in vitro.
MA5-12770 was used in immunocytochemistry and western blot to study the mechanism by which MAGP-1 promotes human ciliary zonule development
|Fujita T,Tsuruga E,Yamanouchi K,Sawa Y,Ishikawa H||Acta histochemica et cytochemica (47:11)||2014|
Matrix metalloproteinase-2 degrades fibrillin-1 and fibrillin-2 of oxytalan fibers in the human eye and periodontal ligaments in vitro.
MA5-12770 was used in immunocytochemistry to study differences in the in vitro ability of MMP-2 to degrade fibrillin-1 and fibrillin-2 from eye and peridontal ligaments
|Kawagoe M,Tsuruga E,Oka K,Sawa Y,Ishikawa H||Acta histochemica et cytochemica (46:153)||2013|
Induction of apoptosis and fibrillin 1 expression in human dermal endothelial cells by scleroderma sera containing anti-endothelial cell antibodies.
MA5-12770 was used in immunocytochemistry to determine the component of scleroderma sera responsible for its effect on dermal endothelial cell apoptosis and fibrillin 1 expression
|Ahmed SS,Tan FK,Arnett FC,Jin L,Geng YJ||Arthritis and rheumatism (54:2250)||2006|
A dermal equivalent developed from fibroblast culture alone: effect of EGF and insulin.
MA5-12770 was used in western blot to examine the role of fibrillin-1 in the in vitro formation of dermal equivalent from cell culture
|Lee DY,Yang JM,Park KH||Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society (15:936)||2007|
fibrillin 15; fibrillin-1; MP340
ACMICD; BOS_10884; ECTOL1; FBN; FBN1; GPHYSD2; MASS; MFLS; MFS1; OCTD; SGS; SSKS; WMS; WMS2