Immunofluorescence analysis of Fibrillin-1 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Fibrillin-1 (11C1.3) Mouse Monoclonal Antibody (MA512770) at 2µg/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Bovine, Human|
|Published species reactivity||Pig, Human, Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Microfibrils from zonular apparatus of bovine eye|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunomicroscopy (IM)||Assay Dependent|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-12770 targets Fibrillin-1 in ICC/IF, IHC (F), IM, and WB applications and shows reactivity with Bovine and Human samples.
The MA5-12770 immunogen is microfibrils from zonular apparatus of bovine eye.
Fibrillin-1 is an extracellular acidic protein with a high cysteine content and an extended thread-like shape with mosaic composition of different types of extracellular modules. Most of the fibrillin molecule is contributed by 47 epidermal growth factor-like (EGF-like) repeats; 43 of them have a consensus sequence for calcium binding (cb). Other modules including 8-cysteine motifs (seven), hybrid motifs (two), and unique domains (three) are interspersed throughout the molecule[3-7]. fibrillin-1 contains a proline-rich region close to the N-terminus that is replaced by a glycine-rich region in fibrillin-2. The fibrillins are found throughout the connective tissue as integral components of extended fibrils.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
GLUT10 deficiency leads to oxidative stress and non-canonical ¿vß3 integrin-mediated TGFß signalling associated with extracellular matrix disarray in arterial tortuosity syndrome skin fibroblasts.
MA5-12770 was used in immunocytochemistry to characterize arterial tortuosity syndrome skin fibroblasts and GLUT10 deficiency leading to non-canonical alpha-gamma-beta3 integrin-mediated TGF-beta singalling and oxidative stress with extracellular matrix d
|Zoppi N,Chiarelli N,Cinquina V,Ritelli M,Colombi M||Human molecular genetics (24:6769)||2015|
Microfibril-associated glycoprotein-1 controls human ciliary zonule development in vitro.
MA5-12770 was used in immunocytochemistry and western blot to study the mechanism by which MAGP-1 promotes human ciliary zonule development
|Fujita T,Tsuruga E,Yamanouchi K,Sawa Y,Ishikawa H||Acta histochemica et cytochemica (47:11)||2014|
Matrix metalloproteinase-2 degrades fibrillin-1 and fibrillin-2 of oxytalan fibers in the human eye and periodontal ligaments in vitro.
MA5-12770 was used in immunocytochemistry to study differences in the in vitro ability of MMP-2 to degrade fibrillin-1 and fibrillin-2 from eye and peridontal ligaments
|Kawagoe M,Tsuruga E,Oka K,Sawa Y,Ishikawa H||Acta histochemica et cytochemica (46:153)||2013|
Induction of apoptosis and fibrillin 1 expression in human dermal endothelial cells by scleroderma sera containing anti-endothelial cell antibodies.
MA5-12770 was used in immunocytochemistry to determine the component of scleroderma sera responsible for its effect on dermal endothelial cell apoptosis and fibrillin 1 expression
|Ahmed SS,Tan FK,Arnett FC,Jin L,Geng YJ||Arthritis and rheumatism (54:2250)||2006|
Geographical ancestry is a key determinant of epidermal morphology and dermal composition.
MA5-12770 was used in immunohistochemistry to study epidermal morphology and dermal composition in individuals of diverese geographic ancestry
|Langton AK,Sherratt MJ,Sellers WI,Griffiths CE,Watson RE||The British journal of dermatology (171:274)||2014|
Latent transforming growth factor-ß binding protein 2 negatively regulates coalescence of oxytalan fibers induced by stretching stress.
MA5-12770 was used in immunohistochemistry to study the mechanism by which LTBP-2 modulates the coalescence of oxytalan fibres
|Tsuruga E,Oka K,Hatakeyama Y,Isokawa K,Sawa Y||Connective tissue research (53:521)||2012|
Tomato paste rich in lycopene protects against cutaneous photodamage in humans in vivo: a randomized controlled trial.
MA5-12770 was used in immunohistochemistry to study the ability of tomato paste rich in lycopene to protect against cutaneous photodamage in humans
|Rizwan M,Rodriguez-Blanco I,Harbottle A,Birch-Machin MA,Watson RE,Rhodes LE||The British journal of dermatology (164:154)||2011|
Effects of a cosmetic 'anti-ageing' product improves photoaged skin [corrected].
MA5-12770 was used in immunohistochemistry to study the effects of a cosmetic 'anti-ageing' product on fibrillin-1 deposition and repair of photoaged skin
|Watson RE,Ogden S,Cotterell LF,Bowden JJ,Bastrilles JY,Long SP,Griffiths CE||The British journal of dermatology (161:419)||2009|
Increased expression of extracellular matrix proteins in rapid atrial pacing-induced atrial fibrillation.
MA5-12770 was used in immunohistochemistry to examine the change of extracellular matrix proteins during atrial fibrillation
|Lin CS,Lai LP,Lin JL,Sun YL,Hsu CW,Chen CL,Mao SJ,Huang SK||Heart rhythm (4:938)||2007|
Photoprotective and anti-skin-aging effects of eicosapentaenoic acid in human skin in vivo.
MA5-12770 was used in immunohistochemistry to investigate the protective effect of eicosapentaenoic acid against UV-induced skin damage and intrinsic aging
|Kim HH,Cho S,Lee S,Kim KH,Cho KH,Eun HC,Chung JH||Journal of lipid research (47:921)||2006|
The effects of a novel synthetic retinoid, seletinoid G, on the expression of extracellular matrix proteins in aged human skin in vivo.
MA5-12770 was used in immunohistochemistry to study the therapeutic benefits of topical application of the synthetic seletinoid G
|Kim MS,Lee S,Rho HS,Kim DH,Chang IS,Chung JH||Clinica chimica acta; international journal of clinical chemistry (362:161)||2005|
Sex steroids modulate human aortic smooth muscle cell matrix protein deposition and matrix metalloproteinase expression.
MA5-12770 was used in immunohistochemistry to study the role of sex steroids in modulating matrix protein deposition and MMP expression in human aortic smooth muscle cells
|Natoli AK,Medley TL,Ahimastos AA,Drew BG,Thearle DJ,Dilley RJ,Kingwell BA||Hypertension (Dallas, Tex. : 1979) (46:1129)||2005|
Ramipril reduces large-artery stiffness in peripheral arterial disease and promotes elastogenic remodeling in cell culture.
MA5-12770 was used in immunohistochemistry to study the mechanisms by which ramipril improves cardiovascular outcome in patients with peripheral arterial disease
|Ahimastos AA,Natoli AK,Lawler A,Blombery PA,Kingwell BA||Hypertension (Dallas, Tex. : 1979) (45:1194)||2005|
Abnormal hepatic expression of fibrillin-1 in children with cholestasis.
MA5-12770 was used in immunohistochemistry to study the expression of fibrillin-1 in children with cholestasis
|Lamireau T,Dubuisson L,Lepreux S,Bioulac-Sage P,Fabre M,Rosenbaum J,Desmoulière A||The American journal of surgical pathology (26:637)||2002|
A dermal equivalent developed from fibroblast culture alone: effect of EGF and insulin.
MA5-12770 was used in western blot to examine the role of fibrillin-1 in the in vitro formation of dermal equivalent from cell culture
|Lee DY,Yang JM,Park KH||Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society (15:936)||2007|