Invitrogen

Fibrillin 1 Monoclonal Antibody (11C1.3)

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Fibrillin 1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Fibrillin-1 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Fibrillin-1 (11C1.3) Mouse Monoclonal Antibody (Product # MA5-12770) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

Fibrillin 1 Antibody in Immunohistochemistry (IHC) — 1 Figure Top-down view of immunohistochemical analysis of the pericellular matrix (PCM). Images are displayed in their physiopathological order of cellular spatial organization. A 1 /B 1 /C 1 /D 1 /E 1 /F 1 , single strings (enframed in green); A 2 /B 2 /C 2 /D 2 /E 2 /F 2 , double strings (enframed in yellow); A 3 /B 3 /C 3 /D 3 /E 3 /E 3 , small clusters (enframed in orange); A 4 /B 4 /C 4 /D 4 /E 4 /F 4 , big clusters (enframed in red). Immunolabelled PCM (depicted in white) for collagen type VI (B 1 -B 4 ), perlecan (C 1 -C 4 ), collagen type III (D 1 -D 4 ), biglycan (E 1 -E 4 ), and fibrillin-1 (F 1 -F 2 ). From single strings to big clusters, a decrease in signal intensity and pericellular definition can be observed for collagen type VI (B 1 -B 4 ), perlecan (C 1 -C 4 ), and biglycan (E 1 -E 4 ). Signal intensity remains constant, however, for collagen type III (D 1 -D 4 ) and fibrillin-1 (F 1 -F 4 ). Twenty-fold magnification; scale bars represent 20 um. Nuclei are stained in blue (DAPI), the stained PCM components are visualized in white and the cell membrane is depicted by black circles. B 1 -B 4 and C 1 -C 4 adapted from Danalache et al. 39 BC, big clusters; DAPI, 4',6-diamidino-2-phenylindole; SC, small clusters [Color figure can be viewed at wileyonlinelibrary.com ]

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 11 publications 11 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

2 µg/mL

View 6 publications 6 publications

Immunomicroscopy (IM)

Assay-dependent

Product Specifications

Species Reactivity

Bovine, Human

Published species

Human, Pig

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

11C1.3

Immunogen

Microfibrils from zonular apparatus of bovine eye

Conjugate

Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

RRID

AB_10988544

Product Specific Information

MA5-12770 targets Fibrillin-1 in ICC/IF, IHC (F), IM, and WB applications and shows reactivity with Bovine and Human samples.

The MA5-12770 immunogen is microfibrils from zonular apparatus of bovine eye.

Target Information

Fibrillin 1 is an extracellular acidic protein with a high cysteine content and an extended thread-like shape with mosaic composition of different types of extracellular modules. Most of the fibrillin molecule is contributed by 47 epidermal growth factor-like (EGF-like) repeats; 43 of them have a consensus sequence for calcium binding (cb). Other modules including 8-cysteine motifs (seven), hybrid motifs (two), and unique domains (three) are interspersed throughout the molecule[3-7]. Fibrillin 1 contains a proline-rich region close to the N-terminus that is replaced by a glycine-rich region in Fibrillin 2. The fibrillins are found throughout the connective tissue as integral components of extended fibrils.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.