Invitrogen

Fibrillin 1 Monoclonal Antibody (11C1.3)

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Fibrillin 1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Fibrillin-1 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Fibrillin-1 (11C1.3) Mouse Monoclonal Antibody (Product # MA5-12770) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

Fibrillin 1 Antibody in Immunohistochemistry (IHC) — 1 Figure Top-down view of immunohistochemical analysis of the pericellular matrix (PCM). Images are displayed in their physiopathological order of cellular spatial organization. A 1 /B 1 /C 1 /D 1 /E 1 /F 1 , single strings (enframed in green); A 2 /B 2 /C 2 /D 2 /E 2 /F 2 , double strings (enframed in yellow); A 3 /B 3 /C 3 /D 3 /E 3 /E 3 , small clusters (enframed in orange); A 4 /B 4 /C 4 /D 4 /E 4 /F 4 , big clusters (enframed in red). Immunolabelled PCM (depicted in white) for collagen type VI (B 1 -B 4 ), perlecan (C 1 -C 4 ), collagen type III (D 1 -D 4 ), biglycan (E 1 -E 4 ), and fibrillin-1 (F 1 -F 2 ). From single strings to big clusters, a decrease in signal intensity and pericellular definition can be observed for collagen type VI (B 1 -B 4 ), perlecan (C 1 -C 4 ), and biglycan (E 1 -E 4 ). Signal intensity remains constant, however, for collagen type III (D 1 -D 4 ) and fibrillin-1 (F 1 -F 4 ). Twenty-fold magnification; scale bars represent 20 um. Nuclei are stained in blue (DAPI), the stained PCM components are visualized in white and the cell membrane is depicted by black circles. B 1 -B 4 and C 1 -C 4 adapted from Danalache et al. 39 BC, big clusters; DAPI, 4',6-diamidino-2-phenylindole; SC, small clusters [Color figure can be viewed at wileyonlinelibrary.com ]

Product Details

MA5-12770

Applications	Tested Dilution	Publications
Western Blot (WB)	1-2 µg/mL	View 1 publication 1 publication
Immunohistochemistry (Frozen) (IHC (F))	Assay-dependent	-
Immunocytochemistry (ICC/IF)	2 µg/mL	View 6 publications 6 publications
Immunomicroscopy (IM)	Assay-dependent	-
Immunohistochemistry (IHC)	-	View 12 publications 12 publications
Immunohistochemistry (Paraffin) (IHC (P))	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Bovine, Human
Published species	Human, Pig
Host/Isotype	Mouse / IgG1, kappa
Class	Monoclonal
Type	Antibody
Clone	11C1.3
Immunogen	Microfibrils from zonular apparatus of bovine eye
Conjugate	Unconjugated
Form	Liquid
Concentration	0.2 mg/mL
Purification	Protein G
Storage buffer	PBS, pH 7.4, with 0.2% BSA
Contains	0.09% sodium azide
Storage conditions	4° C
RRID	AB_10988544

Product Specific Information

MA5-12770 targets Fibrillin-1 in ICC/IF, IHC (F), IM, and WB applications and shows reactivity with Bovine and Human samples.

The MA5-12770 immunogen is microfibrils from zonular apparatus of bovine eye.

Target Information

Fibrillin 1 is an extracellular acidic protein with a high cysteine content and an extended thread-like shape with mosaic composition of different types of extracellular modules. Most of the fibrillin molecule is contributed by 47 epidermal growth factor-like (EGF-like) repeats; 43 of them have a consensus sequence for calcium binding (cb). Other modules including 8-cysteine motifs (seven), hybrid motifs (two), and unique domains (three) are interspersed throughout the molecule[3-7]. Fibrillin 1 contains a proline-rich region close to the N-terminus that is replaced by a glycine-rich region in Fibrillin 2. The fibrillins are found throughout the connective tissue as integral components of extended fibrils.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Targeting feed-forward signaling of TGFβ/NOX4/DHFR/eNOS uncoupling/TGFβ axis with anti-TGFβ and folic acid attenuates formation of aortic aneurysms: Novel mechanisms and therapeutics.

Redox biology

Huang K,Wang Y,Siu KL,Zhang Y,Cai H

MA5-12770 was used in Western Blotting to identify novel mechanisms and therapeutics for thoracic aortic aneurysm in Fbn1C1039G/+ Marfan Syndrome mice.

Fri Jan 01 00:00:00 EST 2021

Biochemical changes of the pericellular matrix and spatial chondrocyte organization-Two highly interconnected hallmarks of osteoarthritis.

Journal of orthopaedic research : official publication of the Orthopaedic Research Society

Danalache M,Erler AL,Wolfgart JM,Schwitalle M,Hofmann UK

MA5-12770 was used in Immunohistochemistry-immunofluorescence to characterize PCM-degradation based on different cellular patterns.

Thu Oct 01 00:00:00 EDT 2020

Biochemical changes of the pericellular matrix and spatial chondrocyte organization-Two highly interconnected hallmarks of osteoarthritis.

Journal of orthopaedic research : official publication of the Orthopaedic Research Society

Danalache M,Erler AL,Wolfgart JM,Schwitalle M,Hofmann UK

MA5-12770 was used in Immunohistochemistry-immunofluorescence to characterize PCM-degradation based on different cellular patterns.

Thu Oct 01 00:00:00 EDT 2020

Transcriptome analysis of skin fibroblasts with dominant negative COL3A1 mutations provides molecular insights into the etiopathology of vascular Ehlers-Danlos syndrome.

PloS one

Chiarelli N,Carini G,Zoppi N,Ritelli M,Colombi M

MA5-12770 was used in Immunohistochemistry to study the molecular mechanisms underlying Vascular Ehlers-Danlos syndrome.

Tue Feb 20 00:00:00 EST 2018

Results from in vitro and ex vivo skin aging models assessing the antiglycation and anti-elastase MMP-12 potential of glycylglycine oleamide.

Clinical, cosmetic and investigational dermatology

Bogdanowicz P,Haure MJ,Ceruti I,Bessou-Touya S,Castex-Rizzi N

MA5-12770 was used in Immunohistochemistry-immunofluorescence to study the in vitro and ex vivo assays in human skin cells and explants and the antiaging effects of glycylglycine oleamide.

Wed Jul 06 00:00:00 EDT 2016

GLUT10 deficiency leads to oxidative stress and non-canonical αvβ3 integrin-mediated TGFβ signalling associated with extracellular matrix disarray in arterial tortuosity syndrome skin fibroblasts.

Human molecular genetics

Zoppi N,Chiarelli N,Cinquina V,Ritelli M,Colombi M

MA5-12770 was used in immunocytochemistry to characterize arterial tortuosity syndrome skin fibroblasts and GLUT10 deficiency leading to non-canonical alpha-gamma-beta3 integrin-mediated TGF-beta singalling and oxidative stress with extracellular matrix d

Tue Dec 01 00:00:00 EST 2015

Geographical ancestry is a key determinant of epidermal morphology and dermal composition.

The British journal of dermatology

Langton AK,Sherratt MJ,Sellers WI,Griffiths CE,Watson RE

MA5-12770 was used in immunohistochemistry to study epidermal morphology and dermal composition in individuals of diverese geographic ancestry

Fri Aug 01 00:00:00 EDT 2014

Microfibril-associated glycoprotein-1 controls human ciliary zonule development in vitro.

Acta histochemica et cytochemica

Fujita T,Tsuruga E,Yamanouchi K,Sawa Y,Ishikawa H

MA5-12770 was used in immunocytochemistry and western blot to study the mechanism by which MAGP-1 promotes human ciliary zonule development

Thu Feb 27 00:00:00 EST 2014

Matrix metalloproteinase-2 degrades fibrillin-1 and fibrillin-2 of oxytalan fibers in the human eye and periodontal ligaments in vitro.

Acta histochemica et cytochemica

Kawagoe M,Tsuruga E,Oka K,Sawa Y,Ishikawa H

MA5-12770 was used in immunocytochemistry to study differences in the in vitro ability of MMP-2 to degrade fibrillin-1 and fibrillin-2 from eye and peridontal ligaments

Wed Oct 30 00:00:00 EDT 2013

Latent transforming growth factor-β binding protein 2 negatively regulates coalescence of oxytalan fibers induced by stretching stress.

Connective tissue research

Tsuruga E,Oka K,Hatakeyama Y,Isokawa K,Sawa Y

MA5-12770 was used in immunohistochemistry to study the mechanism by which LTBP-2 modulates the coalescence of oxytalan fibres

Fri May 03 00:00:00 EDT 2013

Tomato paste rich in lycopene protects against cutaneous photodamage in humans in vivo: a randomized controlled trial.

The British journal of dermatology

Rizwan M,Rodriguez-Blanco I,Harbottle A,Birch-Machin MA,Watson RE,Rhodes LE

MA5-12770 was used in immunohistochemistry to study the ability of tomato paste rich in lycopene to protect against cutaneous photodamage in humans

Sat Jan 01 00:00:00 EST 2011

Effects of a cosmetic 'anti-ageing' product improves photoaged skin [corrected].

The British journal of dermatology

Watson RE,Ogden S,Cotterell LF,Bowden JJ,Bastrilles JY,Long SP,Griffiths CE

MA5-12770 was used in immunohistochemistry to study the effects of a cosmetic 'anti-ageing' product on fibrillin-1 deposition and repair of photoaged skin

Sat Aug 01 00:00:00 EDT 2009

A dermal equivalent developed from fibroblast culture alone: effect of EGF and insulin.

Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society

Lee DY,Yang JM,Park KH

MA5-12770 was used in western blot to examine the role of fibrillin-1 in the in vitro formation of dermal equivalent from cell culture

Fri Mar 07 00:00:00 EST 2008

Increased expression of extracellular matrix proteins in rapid atrial pacing-induced atrial fibrillation.

Heart rhythm

Lin CS,Lai LP,Lin JL,Sun YL,Hsu CW,Chen CL,Mao SJ,Huang SK

MA5-12770 was used in immunohistochemistry to examine the change of extracellular matrix proteins during atrial fibrillation

Sun Jul 01 00:00:00 EDT 2007

Induction of apoptosis and fibrillin 1 expression in human dermal endothelial cells by scleroderma sera containing anti-endothelial cell antibodies.

Arthritis and rheumatism

Ahmed SS,Tan FK,Arnett FC,Jin L,Geng YJ

MA5-12770 was used in immunocytochemistry to determine the component of scleroderma sera responsible for its effect on dermal endothelial cell apoptosis and fibrillin 1 expression

Sat Jul 01 00:00:00 EDT 2006

Photoprotective and anti-skin-aging effects of eicosapentaenoic acid in human skin in vivo.

Journal of lipid research

Kim HH,Cho S,Lee S,Kim KH,Cho KH,Eun HC,Chung JH

MA5-12770 was used in immunohistochemistry to investigate the protective effect of eicosapentaenoic acid against UV-induced skin damage and intrinsic aging

Mon May 01 00:00:00 EDT 2006

The effects of a novel synthetic retinoid, seletinoid G, on the expression of extracellular matrix proteins in aged human skin in vivo.

Clinica chimica acta; international journal of clinical chemistry

Kim MS,Lee S,Rho HS,Kim DH,Chang IS,Chung JH

MA5-12770 was used in immunohistochemistry to study the therapeutic benefits of topical application of the synthetic seletinoid G

Thu Dec 01 00:00:00 EST 2005

Sex steroids modulate human aortic smooth muscle cell matrix protein deposition and matrix metalloproteinase expression.

Hypertension (Dallas, Tex. : 1979)

Natoli AK,Medley TL,Ahimastos AA,Drew BG,Thearle DJ,Dilley RJ,Kingwell BA

MA5-12770 was used in immunohistochemistry to study the role of sex steroids in modulating matrix protein deposition and MMP expression in human aortic smooth muscle cells