Invitrogen

Fibrinogen Recombinant Polyclonal Antibody (12 HCLC)

View all (44) Fibrinogen antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

Fibrinogen Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Fibrinogen showing staining in the cytoplasm of paraffin-embedded human kidney tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Fibrinogen Recombinant Rabbit Polyclonal Antibody (Product # 710386) diluted in 3% BSA-PBS at a dilution of 1:100 for 1 hour at 37ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Fibrinogen Antibody in Western Blot (WB) — Western blot was performed using Anti-Fibrinogen Polyclonal Antibody (Product # 710386 ) and bands around 70 and 51 kDa corresponding to Fibrinogen alpha and gamma chain were observed in Human Plasma. 10 µl of Human Plasma was electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 0.25 µg/mL, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Product Details

710386

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Immunohistochemistry (Paraffin) (IHC (P))

1:100

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

EXPI293

Class

Recombinant Polyclonal

Type

Antibody

Clone

12 HCLC

Immunogen

Full length peptide of human Fibrinogen

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2532701

Product Specific Information

This antibody is predicted to react with non-human primate based on sequence homology.

Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.

Target Information

Fibrinogen, the precursor of fibrin, is the coagulable protein in the blood plasma of vertebrates. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. Fibrinogen consists as a dimer of 3 pairs of non-identical chains FGA, FGB, and FGG that are cross-linked by disulfide bonds in their N-terminal segments. The molecule has 2 terminal D domains and one central E domain, all three domains are separated when fibrinogen is degradated by plasmin. Mutations in this gene lead to several disorders, including dysfibrinogenemia, afibrinogenemia, and renal amyloidosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: epididymis secretory sperm binding protein Li 78p; fibg; Fibrinogen alpha chain; Fibrinogen beta chain; Fibrinogen gamma chain; fibrinogen, A alpha polypeptide; fibrinogen, B beta polypeptide; fibrinogen, gamma polypeptide; Fibrinopeptide A; Fibrinopeptide B; MGC119422; MGC119423; MGC119425; testicular tissue protein Li 70

Gene Aliases: FGA; FGB; FGG; Fib2; HEL-S-78p; PRO2061

UniProt ID: (Human) P02671, (Human) P02675, (Human) P02679

Entrez Gene ID: (Human) 2243, (Human) 2244, (Human) 2266

Molecular Function: intercellular signal molecule