|Tested species reactivity||Human, Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rat / IgG2a|
|Storage buffer||PBS with 0.1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:50|
|Immunohistochemistry (Frozen) (IHC (F))||1:50|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
MA1-40076 detects Fibroblasts from mouse samples. MA1-40076 is cross reactive with human tonsil fibroblasts. It does not cross react with purified laminin, collagen types I-V, fibronectin, heparin sulfate proteoglycan,entactin or nidogen.
MA1-40076 has been successfully used in immunohistochemistry (frozen and parrafin), flow cytometry, and immunofluorescence applications. Suggested positive control is spleen
The MA1-40076 immunogen is mouse thymic stromal cells.
Fibroblast are the least specialized cells in the connective-tissue family. Thay are dispersed in connective tissue throughout the body, where they secrete a nonrigid extracellular matrix that is rich in type I and/or type III collagen. The activation of fibroblasts by inflammatory stimuli results in their migration, proliferation and deposition of extracellular matrix components, important features involved in both wound healing and fibrosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Knock-in reporter mice demonstrate that DNA repair by non-homologous end joining declines with age.
MA1-40076 was used in immunocytochemistry to examine non-homologous end joining in aging mice
|Vaidya A,Mao Z,Tian X,Spencer B,Seluanov A,Gorbunova V||PLoS genetics (10:null)||2014|