Western blot analysis of Fibronectin was performed by loading 50 ug of human serum lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with a Fibronectin monoclonal antibody (Product # MA5-12314) at a dilution of 1:50 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated goat anti-mouse IgG + IgM (H+L) cross-adsorbed secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~260 kDa.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human fibronectin purified from serum by affinity chromatography on gelatin-sepharose|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1:10-1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-12314 targets Fibronectin in IF, IP, and WB applications and shows reactivity with Human samples.
The MA5-12314 immunogen is human fibronectin purified from serum by affinity chromatography on gelatin-sepharose.
Fibronectin is a dimeric glycoprotein which is present in cells, extracellular matrix, and blood. It possesses at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectin is involved in cell adhesion, tissue organization, and wound healing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Extracellular Fibrinogen-binding Protein (Efb) from Staphylococcus aureus Inhibits the Formation of Platelet-Leukocyte Complexes.
MA5-12314 was used in western blot to elucidate how the extracellular fibrinogen-binding protein from Staphylococcus aureus inhibits platelet activation
|Posner MG,Upadhyay A,Abubaker AA,Fortunato TM,Vara D,Canobbio I,Bagby S,Pula G||The Journal of biological chemistry (291:2764)||2016|
Multipotent stem cells are effectively collected from adult human cheek skin.
MA5-12314 was used in immunocytochemistry to study adult human cheek skin as an attractive source of multipotent stem cells
|Yoshikawa K,Naitoh M,Kubota H,Ishiko T,Aya R,Yamawaki S,Suzuki S||Biochemical and biophysical research communications (431:104)||2013|
LC3-mediated fibronectin mRNA translation induces fibrosarcoma growth by increasing connective tissue growth factor.
MA5-12314 was used in western blot to examine the role of fibronectin mRNA translation in the growth of fibrosarcoma
|Ying L,Lau A,Alvira CM,West R,Cann GM,Zhou B,Kinnear C,Jan E,Sarnow P,Van de Rijn M,Rabinovitch M||Journal of cell science (122:1441)||2009|
FAK-independent alphavbeta3 integrin-EGFR complexes rescue from anoikis matrix-defective fibroblasts.
MA5-12314 was used in blocking or activating experiment to study the role of FAK-independent alphavbeta3 integrin-EGFR complexes in rescuing matrix-defective fibroblasts from anoikis
|Zoppi N,Barlati S,Colombi M||Biochimica et biophysica acta (1783:1177)||2008|