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Immunofluorescence analysis of Filaggrin was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Filaggrin (FLG01) Mouse Monoclonal Antibody (Product # MA5-13440) at 2µg/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjµgate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant filaggrin protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||2-4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-13440 targets Filaggrin in IHC (P) applications and shows reactivity with Human samples.
The MA5-13440 immunogen is recombinant filaggrin protein.
Filaggrin is expressed only in well differentiated keratinized epithelial cells. The synthesis of filaggrin varies in types of ichthyosis, and with viral, premalignant and malignant conditions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Mutations in SERPINB7, encoding a member of the serine protease inhibitor superfamily, cause Nagashima-type palmoplantar keratosis.
MA5-13440 was used in immunohistochemistry to study Nagashima-type palmoplantar keratosis caused by putative loss-of-function mutations in SERPINB7
|Kubo A,Shiohama A,Sasaki T,Nakabayashi K,Kawasaki H,Atsugi T,Sato S,Shimizu A,Mikami S,Tanizaki H,Uchiyama M,Maeda T,Ito T,Sakabe J,Heike T,Okuyama T,Kosaki R,Kosaki K,Kudoh J,Hata K,Umezawa A,Tokura Y,Ishiko A,Niizeki H,Kabashima K,Mitsuhashi Y,Amagai M||American journal of human genetics (93:945)||2013|
Pituitary tumor transforming gene 1 induces tumor necrosis factor-α production from keratinocytes: implication for involvement in the pathophysiology of psoriasis.
MA5-13440 was used in immunocytochemistry and immunohistochemistry to study the induction of keratinocyte TNF-alpha production by the PTTG1 transcription factor and the significance for psoriasis
|Ishitsuka Y,Kawachi Y,Maruyama H,Taguchi S,Fujisawa Y,Furuta J,Nakamura Y,Ishii Y,Otsuka F||The Journal of investigative dermatology (133:2566)||2013|
Saurian papulosis: a new clinicopathological entity.
MA5-13440 was used in immunohistochemistry to characterize two clinical cases of saurian papulosis
|Molina-Ruiz AM,del Carmen Fariña M,Carrasco L,Santonja C,Rodríguez-Peralto JL,Torrelo A,Kutzner H,Requena L||Journal of the American Academy of Dermatology (68:e17)||2013|
Papillary fibroblasts differentiate into reticular fibroblasts after prolonged in vitro culture.
MA5-13440 was used in immunohistochemistry to study the propensity of papillary fibroblasts to develop a reticular fibroblast phenotype following prolonged passage in vitro
|Janson D,Saintigny G,Mahé C,El Ghalbzouri A||Experimental dermatology (22:48)||2013|
Characterization of multipotent cells from human adult hair follicles.
MA5-13440 was used in immunohistochemistry to characterize the multipotent endothelial cells from stripped human hair follicle
|Raposio E,Guida C,Baldelli I,Curto M,Fiocca R,Kunkl A,Robello G,Santi PL||Toxicology in vitro : an international journal published in association with BIBRA (21:320)||2007|
A characteristic subset of psoriasis-associated genes is induced by oncostatin-M in reconstituted epidermis.
MA5-13440 was used in immunohistochemistry to study the role of oncostatin-M in inducing a characteristic subset of psoriasis-associated genes in reconstituted epidermis
|Gazel A,Rosdy M,Bertino B,Tornier C,Sahuc F,Blumenberg M||The Journal of investigative dermatology (126:2647)||2006|
A decisive function of transforming growth factor-β/Smad signaling in tissue morphogenesis and differentiation of human HaCaT keratinocytes.
MA5-13440 was used in immunocytochemistry to study the role of TGF-beta/Smad signaling in tissue morphogenesis and differentiation of human HaCaT keratinocytes
|Buschke S,Stark HJ,Cerezo A,Prätzel-Wunder S,Boehnke K,Kollar J,Langbein L,Heldin CH,Boukamp P||Molecular biology of the cell (22:782)||2011|
Establishment and characterization of immortalized human gingival keratinocyte cell lines.
MA5-13440 was used in flow cytometry to establish and characterize the immortalized human gingival keratinocyte cell lines
|Gröger S,Michel J,Meyle J||Journal of periodontal research (43:604)||2008|