|Tested species reactivity||Luciferase|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant luciferase expressed in E. Coli|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||10 ug/ml|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Western Blot (WB)||1/100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Membrane permeabilization is required for flow cytometry applications. In Western Blot , MA1-80225 detects a band at ~55-60 kDa using 1 ug of purified luciferase. For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
Luciferase is a luminescent enzyme, mostly known for its role as a reporter protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.