Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
Western blot analysis of Firefly Luciferase using 30µg of A) 293T (B) 293T transfected firefly and C) 293T transfected Renilla Luciferase lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a Firefly Luciferase polyclonal antibody (Product # PA5-32208) at a dilution of 1:5000.
|Tested species reactivity||Luciferase|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment contain a sequence corresponding to a region within amino acids 1 and 550 of Firefly Luciferase|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-32208 targets Firefly Luciferase in WB applications and shows reactivity with Firefly samples.
The PA5-32208 immunogen is a recombinant fragment corresponding to a region within amino acids 1 and 550 of Firefly Luciferase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.