Membrane permeabilization is required for flow cytometry applications. In Western Blot , MA1-80225 detects a band at ~55-60 kDa using 1 µg of purified luciferase. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
Mouse anti Luciferase antibody, clone LUC-Y detects the expression of the luciferase gene of the North American firefly Photinus pyralis in cells and tissues.
Luciferase produces green light with the wavelength of 562 nm. Luciferase catalyzes the reaction: D-firefly luciferin + O2 + ATP = firefly oxyluciferin + CO2 + AMP + diphosphate + light.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Firefly Luciferase; Genji firefly; Green Luciferase; Japanese firefly; luciferin 4-monooxygenase; Photinus pyralis; photinus pyralis luciferase