Immunohistochemistry of methcarn-fixed, paraffin-embedded hippocampus from control (normal) and Alzheimer brain using a Fis1 polyclonal antibody (Product # PA1-41082). Quantification in five AD brains and five age-matched controls indicated that Fis1 was detected in only the soma in ~80% of pyramidal neurons in AD hippocampus, significantly different from what was seen for control hippocampal neurons.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues 11-25 of human Fis1.|
|Storage buffer||whole serum diluted in PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
This antibody is predicted to react with rat, chimp, and canine based on sequence homology.
Suggested positive control: antigen standard for FIS1 (transient overexpression lysate), 3T3 or Hela whole cell lysate.
Fis1, a 17KDa integral membrane protein, is a novel member of mitochondrial fission machinery. It consists of a central leucine-zipper domain, a coiled-coil region, a tetratricopeptide repeat domain and a C-terminal transmembrane domain. It is a human orthologue of Fis1p (S.cerevisiae) and is localized in the outer mitochondrial membrane. Fis1 acts as a pro-apoptotic factor and plays a vital role in the regulation of mitochondrial fission. It interacts with DLP1 and Mdv1p and facilitates Dnm1p recruitment to the functional fission complex, thereby inducing apoptosis. Role of Fis1 has also been implicated in growth and division of peroxisomes. Loss of Fis1 inhibits apoptosis and results in elongation of mammalian mitochondria.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Autophagy signaling in skeletal muscle of infarcted rats.
PA1-41082 was used in western blot to study skeletal muscle atrophy induced by myocardial infarction and the role of autophagy signaling
|Jannig PR,Moreira JB,Bechara LR,Bozi LH,Bacurau AV,Monteiro AW,Dourado PM,Wisløff U,Brum PC||PloS one (9:null)||2014|