Western blot analysis of FLI-1 was performed by loading 20ug of indicated whole cell lysates and 10ul PageRuler Plus Prestained Protein Ladder (Product # 26619) per well onto a 4-20% Tris-Glycine polyacrylamide gel (Product # WT4202). Proteins were transferred to a nitrocellulose membrane using the G2 Fast Blotter (Product # 62288) and blocked with 5% Milk/TBST for at least 1 hour at room temperature. FLI-1 was detected at ~54kD using a FLI-1 rabbit polyclonal antibody (Product # PA5-16899) at a concentration of 2ug/ml in blocking buffer overnight at 4°C on a rocking platform, followed by a mouse anti-rabbit light chain specific IgG-HRP secondary antibody at a dilution of 1:10,000 for at least 1 hour at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from C-terminus of human Fli-1|
|Storage buffer||PBS, pH 7.6, with 0.2% BSA|
|Contains||15mM sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Western Blot (WB)||2-4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-16899 targets Fli-1 in WB applications and shows reactivity with Human, mouse, and Rat samples.
The PA5-16899 immunogen is synthetic peptide derived from C-terminus of human Fli-1.
Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. This family of genes currently includes Ets-1, Ets-2, Erg-1, Erg-2, Elk, E74, Fli-1, PU.1 and PEA3. Members of the Ets gene family exhibit varied pattern of tissue expression, and share a highly conserved carboxy terminal domain containing a sequence related to the SV40 large T antigen nuclear localizartion signal sequence.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.